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Literature summary for 5.1.1.18 extracted from

  • Marchetti, M.; Bruno, S.; Campanini, B.; Peracchi, A.; Mai, N.; Mozzarelli, A.
    ATP binding to human serine racemase is cooperative and modulated by glycine (2013), FEBS J., 280, 5853-5863.
    View publication on PubMed
Search for more literature for 5.1.1.18

Activating Compound

Activating Compound Comment Organism Structure
ATP ATP binding to human serine racemase is strongly cooperative and modulated by glycine, the active-site ligand increases the serine racemase affinity for ATP by about 22fold, abolishing cooperativity. ATP increases the noncooperative glycine binding15fold Homo sapiens
glycine glycine stabilizes a protein conformation that binds ATP non-cooperatively and with high affinity, the active-site ligand increases the serine racemase affinity for ATP by about 22fold, abolishing cooperativity. ATP increases the noncooperative glycine binding 15fold Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, kinetics of serine racemase alpha- and beta-elimination activities, overview Homo sapiens
40
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens
48
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Homo sapiens
-
 D-serine
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by metal affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine
-
 Homo sapiens L-serine
-
 r
L-serine
-
 Homo sapiens D-serine
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.048
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens
0.17
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Homo sapiens

General Information

General Information Comment Organism
physiological function through cross-talk between allosteric and active sites, intracellular ATP and glycine control D-serine homeostasis, and, indirectly, NMDA receptor activity. The N-methyl D-aspartate (NMDA) receptors play a key role in excitatory neurotransmission, and control learning, memory and synaptic plasticity. Their activity is modulated by the agonist glutamate and by the co-agonists D-serine and glycine Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
 L-serine in absence of ATP, pH 8.0, 37°C Homo sapiens
0.0042
-
 L-serine in presence of ATP, pH 8.0, 37°C Homo sapiens