Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
E219A/D225A | neither the serine racemase nor the dehydratase activities of the E219A/D225A serine racemase mutant are affected by the addition of Mg2+. The kcat/Km values of the mutant decrease to 16% (for L-Ser) and 23% (for D-Ser) of those of the wild type protein in the racemase reaction and to 36% (for L-Ser) and 26% (for D-Ser) in the dehydratase reaction | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | D-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
8.9 | - |
D-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
10 | - |
L-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
18 | - |
L-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
40 | - |
D-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
48 | - |
D-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
78 | - |
L-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
130 | - |
L-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for serine racemase activity, Glu219 and Asp225 are the essential amino acid residues for Mg2+ to affect both kinds of enzyme activities (racemase and dehydratase) | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Oryza sativa |
80200 | - |
gel filtration | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine | racemization reaction | Oryza sativa | L-serine | - |
r | |
L-serine | racemization reaction | Oryza sativa | D-serine | - |
r | |
additional information | serine racemase is a bifunctional enzyme with racemase and dehydratase activities towards D- and L-serine | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39000, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
SerR | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.14 | - |
D-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.2 | - |
D-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.26 | - |
L-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.31 | - |
D-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.31 | - |
L-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.36 | - |
L-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.42 | - |
L-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
D-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.003 | - |
D-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.0032 | - |
L-serine | mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.0033 | - |
L-serine | mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.013 | - |
D-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.02 | - |
L-serine | wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.022 | - |
D-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa | |
0.031 | - |
L-serine | wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication | Oryza sativa |