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Literature summary for 5.1.1.18 extracted from

  • Gogami, Y.; Kobayashi, A.; Ikeuchi, T.; Oikawa, T.
    Site-directed mutagenesis of rice serine racemase: evidence that Glu219 and Asp225 mediate the effects of Mg2+ on the activity (2010), Chem. Biodivers., 7, 1579-1590.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Protein Variants

Protein Variants Comment Organism
E219A/D225A neither the serine racemase nor the dehydratase activities of the E219A/D225A serine racemase mutant are affected by the addition of Mg2+. The kcat/Km values of the mutant decrease to 16% (for L-Ser) and 23% (for D-Ser) of those of the wild type protein in the racemase reaction and to 36% (for L-Ser) and 26% (for D-Ser) in the dehydratase reaction Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 D-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
8.9
-
D-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
10
-
L-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
18
-
L-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
40
-
D-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
48
-
D-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
78
-
L-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
130
-
L-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for serine racemase activity, Glu219 and Asp225 are the essential amino acid residues for Mg2+ to affect both kinds of enzyme activities (racemase and dehydratase) Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Oryza sativa
80200
-
gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine racemization reaction Oryza sativa L-serine
-
r
L-serine racemization reaction Oryza sativa D-serine
-
r
additional information serine racemase is a bifunctional enzyme with racemase and dehydratase activities towards D- and L-serine Oryza sativa ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 39000, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
SerR
-
Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
D-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.14
-
D-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.2
-
D-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.26
-
L-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.31
-
D-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.31
-
L-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.36
-
L-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.42
-
L-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
D-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.003
-
D-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.0032
-
L-serine mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.0033
-
L-serine mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.013
-
D-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.02
-
L-serine wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication Oryza sativa
0.022
-
D-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa
0.031
-
L-serine wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication Oryza sativa