Literature summary for 5.1.1.18 extracted from

Yamauchi, T.; Goto, M.; Wu, H.Y.; Uo, T.; Yoshimura, T.; Mihara, H.; Kurihara, T.; Miyahara, I.; Hirotsu, K.; Esaki, N.
Serine racemase with catalytically active lysinoalanyl residue (2009), J. Biochem., 145, 421-424.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
native and modified enzyme, X-ray diffraction structure determination and analysis at 1.7 A resolution	Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
additional information	the yeast enzyme is covalently modified with dehydroalanine derived from its natural substrate serine, giving a serine racemase with catalytically active lysinoalanyl residue. The modification is not reversed by incubation in 20 mM potassium phosphate buffer, pH 7.2, without serine for several days at room temperature. The enzyme remains partially active even though its essential Lys57 inherently forming a Schiff base with the coenzyme pyridoxal 5'-phosphate is converted to N(6)-(R-2-amino-2-carboxyethyl)-L-lysyl (lysino-D-alanyl) residue. The alpha-amino group of the D-alanyl moiety of the lysino-D-alanyl residue serves as a catalytic base in the same manner asthe epsilon-amino group of Lys57 of the original enzyme. The specific activities of modified spSR for L-serine racemization and L-serine dehydration are 54%, and 68%, respectively, of those of the original spSR	Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine	Schizosaccharomyces pombe	-	D-serine	-	?

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	O59791	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-serine = D-serine	reaction mechanism, overview	Schizosaccharomyces pombe	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine	-	Schizosaccharomyces pombe	D-serine	-	?

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Schizosaccharomyces pombe

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Release 2023.1 (January 2023)