Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme requires pyridoxal 5'-phosphate and divalent cations such as Ca2+, Mg2+, or Mn2+, but not ATP | Hordeum vulgare |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | complete inhibition of racemase activity at 1 mM | Hordeum vulgare | |
additional information | no inhibition by EDTA of the racemase activity | Hordeum vulgare | |
Ni2+ | - |
Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 3.8fold at 1 mM | Hordeum vulgare | |
Mg2+ | activates 2.9fold at 1 mM, ATP reduces the activation by Mg2+ | Hordeum vulgare | |
Mn2+ | activates 3.8fold at 1 mM | Hordeum vulgare | |
additional information | the enzyme requires pyridoxal 5'-phosphate and divalent cations such as Ca2+, Mg2+, or Mn2+, but not ATP, effects of metal ions on the enzyme's dehydration activity, overview | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35700 | - |
x * 35700, about, sequence calculation, x * 36000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
36000 | - |
x * 35700, about, sequence calculation, x * 36000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Hordeum vulgare | - |
D-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | A5HUN0 | cv. Haruna nijo | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Hordeum vulgare | D-serine | - |
r | |
additional information | the bifunctional enzyme catalyzes not only serine racemization but also dehydration of serine to pyruvate | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35700, about, sequence calculation, x * 36000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | bound by Lys67, Phe66, Gly246, Ser322, and Asn94 | Hordeum vulgare |