Cloned (Comment) | Organism |
---|---|
gene srr, DNA and amino acid sequence determination and anaylsis, expression in Escherichia coli | Pyrobaculum islandicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 10-20% inhibition; 10-20% inhibition at 0.1-1 mM | Pyrobaculum islandicum | |
Cu2+ | 30-40% inhibition | Pyrobaculum islandicum | |
Fe2+ | 20-30% inhibition; 20-30% inhibition | Pyrobaculum islandicum | |
hydroxylamine | complete inhibition at 1 mM | Pyrobaculum islandicum | |
additional information | no inhibition by p-chloromercuribenzoate, iodoacetamide, and iodoacetate | Pyrobaculum islandicum | |
Sodium borohydride | complete inhibition at 1 mM | Pyrobaculum islandicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme shows no allosteric properties, it is not affected by either L-isoleucine or L-valine, or most of the metal ions | Pyrobaculum islandicum | |
additional information | - |
additional information | enzyme shows no allosteric properties | Pyrobaculum islandicum | |
95.4 | - |
D-serine | pH 8.2, 95°C | Pyrobaculum islandicum | |
185 | - |
L-serine | pH 8.2, 95°C | Pyrobaculum islandicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Pyrobaculum islandicum | |
Cu2+ | 30-40% stimulation | Pyrobaculum islandicum | |
Mg2+ | activates | Pyrobaculum islandicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, native enzyme, SDS-PAGE | Pyrobaculum islandicum |
44000 | - |
3 * 44000, SDS-PAGE | Pyrobaculum islandicum |
130000 | - |
gel filtration | Pyrobaculum islandicum |
137000 | - |
native enzyme, gel filtration | Pyrobaculum islandicum |
143000 | - |
native enzyme, light scattering-refraction | Pyrobaculum islandicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Pyrobaculum islandicum | - |
D-serine | - |
r | |
L-threonine | Pyrobaculum islandicum | - |
D-threonine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | - |
bifunctional enzyme, shows additional L-serine/L-threonine dehydratase activity | - |
Pyrobaculum islandicum | Q2V0H1 | anaerobic hyperthermophilic archaeon, strain DSM 4184, gene srr | - |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange chromatography, recombinant enzyme from Escherichia coli by treatment with L-arginine and anion exchange chromatography | Pyrobaculum islandicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth temperature of the organism is 100°C | Pyrobaculum islandicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.6 | - |
pH 8.2, 95°C | Pyrobaculum islandicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine | 38% of the activity with L-serine | Pyrobaculum islandicum | L-serine | - |
r | |
D-threonine | 13% of the activity with L-serine | Pyrobaculum islandicum | L-threonine | - |
r | |
L-serine | - |
Pyrobaculum islandicum | D-serine | - |
r | |
L-serine | the forward reaction is preferred, serine is preferred to threonine | Pyrobaculum islandicum | D-serine | - |
r | |
L-threonine | - |
Pyrobaculum islandicum | D-threonine | - |
r | |
L-threonine | the forward reaction is preferred, serine is preferred to threonine | Pyrobaculum islandicum | D-threonine | - |
r | |
L-threonine | 40% of the activity with L-serine | Pyrobaculum islandicum | D-threonine | - |
r | |
additional information | substrate specificity, the bifunctional enzyme shows high L-serine/L-threonine dehydratase activity, overview | Pyrobaculum islandicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, native enzyme, SDS-PAGE | Pyrobaculum islandicum |
trimer | 3 * 44000, SDS-PAGE | Pyrobaculum islandicum |
Synonyms | Comment | Organism |
---|---|---|
SRR | - |
Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrobaculum islandicum |
95 | - |
both racemase and dehydratase activity | Pyrobaculum islandicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5-phosphate, 95.2% remaining activity | Pyrobaculum islandicum |
75 | - |
8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5-phosphate, 67.1% remaining activity | Pyrobaculum islandicum |
95 | - |
8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5-phosphate, 29.8% remaining activity | Pyrobaculum islandicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.22 | - |
D-serine | pH 8.2, 95°C | Pyrobaculum islandicum | |
14.8 | - |
L-serine | pH 8.2, 95°C | Pyrobaculum islandicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Pyrobaculum islandicum |
8.2 | - |
racemase activity | Pyrobaculum islandicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pyrobaculum islandicum | |
pyridoxal 5'-phosphate | dependent on | Pyrobaculum islandicum |