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Literature summary for 5.1.1.17 extracted from

  • Ullan, R.V.; Casqueiro, J.; Naranjo, L.; Vaca, I.; Martin, J.F.
    Expression of cefD2 and the conversion of isopenicillin N into penicillin N by the two-component epimerase system are rate-limiting steps in cephalosporin biosynthesis (2004), Mol. Genet. Genomics, 272, 562-570.
    View publication on PubMed
Search for more literature for 5.1.1.17

Application

Application Comment Organism
synthesis epimerization is a rate-limiting step in cephalosporin biosynthesis in Acremonium chrysogenum. When epimerase activity is enhanced, there is a clear increase in cephalosporin production. Thus, this method provides a strategy that could easily be applied to improve industrial cephalosporin producing strains Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopenicillin N Acremonium chrysogenum
-
 penicillin N
-
 ?
isopenicillin N Acremonium chrysogenum C10 / ATCC 48272
-
 penicillin N
-
 ?

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
 and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474
-
Acremonium chrysogenum C10 / ATCC 48272
-
 and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Acremonium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 strain C10 (ATCC 48272): 93 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD2: no activity, transformant strain TMCD26: 223 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD32: no activity, transformant strain TMCD39: no activity, transformant strain TMCD53: 226 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD242: 234 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD474: 202 ng of cephalosporin C formed per min and mg of protein Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopenicillin N
-
 Acremonium chrysogenum penicillin N
-
 ?
isopenicillin N
-
 Acremonium chrysogenum C10 / ATCC 48272 penicillin N
-
 ?

Synonyms

Synonyms Comment Organism
IPN epimerase
-
 Acremonium chrysogenum
isopenicillin N-CoA epimerase
-
 Acremonium chrysogenum