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Literature summary for 5.1.1.17 extracted from
- Partial purification and characterization of isopenicillin N epimerase activity from Streptomyces clavuligerus (1983), Can. J. Microbiol., 29, 1526-1531.
General Stability
| General Stability | Organism |
|---|---|
| unstable enzyme | Streptomyces clavuligerus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 10 mM, 68% inhibition, readdition of pyridoxal 5-phosphate causes an increase in activity to 63% of the control | Streptomyces clavuligerus |  |
| Iproniazid | 10 mM, 33% inhibition | Streptomyces clavuligerus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
- |
Streptomyces clavuligerus |
| 60000 | - |
gel filtration | Streptomyces clavuligerus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopenicillin N | Streptomyces clavuligerus | - |
penicillin N | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-200, DEAE-trisacryl | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
19.53 units, 1 unit is defined as the amount of enzyme which produces 0.001 mg of penicillin N in 1 h | Streptomyces clavuligerus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -65°C, 1 week, 61% loss of activity | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopenicillin | - |
Streptomyces clavuligerus | penicillin N | - |
? | |
| isopenicillin N | - |
Streptomyces clavuligerus | penicillin N | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Streptomyces clavuligerus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | putative cofactor, does not increase enzyme activity in assay system, stabilizes enzyme during purification | Streptomyces clavuligerus | |
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