Literature summary for 5.1.1.15 extracted from

Okazaki, S.; Suzuki, A.; Mizushima, T.; Kawano, T.; Komeda, H.; Asano, Y.; Yamane, T.
The novel structure of a pyridoxal 5-phosphate-dependent fold-type I racemase, alpha-amino-epsilon-caprolactam racemase from Achromobacter obae (2009), Biochemistry, 48, 941-950.

Search for more literature for 5.1.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Achromobacter obae

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 20Â°C using the hanging-drop vapor diffusion method, crystal structures of native and epsilon-caprolactam complexed ACLR are solved at 2.21 and 2.40 Ã indicating the catalytic residue as Tyr137	Achromobacter obae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alpha-Amino-epsilon-caprolactam	Achromobacter obae	-	D-alpha-Amino-epsilon-caprolactam	-	r

Organism

Organism	UniProt	Comment	Textmining
Achromobacter obae	Q7M181	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni Sepharose highperformance (Amersham) and anion-exchange chromatography (HiTrapQFF)	Achromobacter obae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alpha-Amino-epsilon-caprolactam	-	Achromobacter obae	D-alpha-Amino-epsilon-caprolactam	-	r

Synonyms

Synonyms	Comment	Organism
ACLR	-	Achromobacter obae
alpha-Amino-epsilon-caprolactam racemase	-	Achromobacter obae

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Release 2023.1 (January 2023)