General Stability | Organism |
---|---|
freezing inactivates, particularly in dilute solution of less than 1 mg/ml | Achromobacter obae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Methyl-3-benzothiazolonehydrazone hydrochloride | - |
Achromobacter obae | |
Ala | weak | Achromobacter obae | |
CuSO4 | - |
Achromobacter obae | |
D-cycloserine | - |
Achromobacter obae | |
epsilon-Caprolactam | - |
Achromobacter obae | |
Glu | weak | Achromobacter obae | |
Gly | - |
Achromobacter obae | |
HgCl2 | - |
Achromobacter obae | |
hydroxylamine | - |
Achromobacter obae | |
L-His | - |
Achromobacter obae | |
L-Lys | - |
Achromobacter obae | |
L-Met | - |
Achromobacter obae | |
L-Orn | - |
Achromobacter obae | |
Leu | weak | Achromobacter obae | |
NEM | - |
Achromobacter obae | |
PCMB | - |
Achromobacter obae | |
phenylhydrazine | - |
Achromobacter obae | |
Pro | weak | Achromobacter obae | |
Sodium borohydride | - |
Achromobacter obae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
L-alpha-amino-epsilon-caprolactam | - |
Achromobacter obae | |
8 | - |
D-alpha-Amino-epsilon-caprolactam | - |
Achromobacter obae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Achromobacter obae | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 49000, SDS-PAGE | Achromobacter obae |
51000 | - |
gel filtration | Achromobacter obae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter obae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Achromobacter obae |
Storage Stability | Organism |
---|---|
-20°C, 0.25 M sucrose, 10 mM potassium phosphate buffer, pH 7.3, 0.02 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, protein concentration: more than 0.3%, stable for at least 6 months | Achromobacter obae |
20°C, 0.25 M sucrose, enzyme retains approximately 90% of its initial activity after storage for 4 months | Achromobacter obae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-Amino-epsilon-caprolactam | r | Achromobacter obae | D-alpha-Amino-epsilon-caprolactam | - |
? | |
additional information | the enzyme catalyzes alpha-proton exchange of the substrate with deuterium during racemization in deuterium oxide | Achromobacter obae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, SDS-PAGE | Achromobacter obae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 7.2, 20 min, stable | Achromobacter obae |
65 | - |
pH 7.2, 20 min, 5% loss of activity | Achromobacter obae |
70 | - |
pH 7.2, 20 min, 15% loss of activity | Achromobacter obae |
75 | - |
pH 7.2, 20 min, 40% loss of activity | Achromobacter obae |
80 | - |
pH 7.2, 20 min, 25% loss of activity | Achromobacter obae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
L-alpha-amino-epsilon-caprolactam | Achromobacter obae |
8.8 | - |
D-alpha-amino-epsilon-caprolactam | Achromobacter obae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | at 25°C, 20 min, stable | Achromobacter obae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme contains 1 mol of pyridoxal 5'-phosphate per mol of enzyme. Km: 0.00021 mM | Achromobacter obae |