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Literature summary for 5.1.1.13 extracted from
- Aspartate racemase, generating neuronal D-aspartate, regulates adult neurogenesis (2010), Proc. Natl. Acad. Sci. USA, 107, 3175-3179.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme is cloned into pET vector, which is transformed into BL21 Escherichia coli | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K136W | the importance of lysine-136, the presumed proton donor, is highlighted by its mutation to tryptophan, which virtually abolishes racemase activity | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amino-oxyacetic acid | - |
Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
L-aspartate | recombinant enzyme | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | Mus musculus | - |
D-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged aspartate racemase is purified on Talon metal affinity resin | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Mus musculus | - |
| brain | - |
Mus musculus | - |
| cerebral cortex | - |
Mus musculus | - |
| chromaffin cell | - |
Mus musculus | - |
| dentate gyrus | - |
Mus musculus | - |
| heart | - |
Mus musculus | - |
| hippocampus | - |
Mus musculus | - |
| additional information | negligible expression in liver, lung and kidney | Mus musculus | - |
| neuroendocrine cell | - |
Mus musculus | - |
| paraventricular nucleus of hypothalamus | - |
Mus musculus | - |
| pinealocyte | - |
Mus musculus | - |
| pyramidal neuron | - |
Mus musculus | - |
| stellate cell | - |
Mus musculus | - |
| supraoptic nucleus | - |
Mus musculus | - |
| testis | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 460 | - |
Vmax of recombinant enzyme, activity is assayed in 500 nl of pyrydoxal 5'-phosphate (100 nM), 0.49 ml of recombinant enzyme (0.5 mg/ml), and 0.01 mL of 1 mM L-aspartate at 37°C for 1 h in 10 mM Tris (pH 7.5) | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | - |
Mus musculus | D-aspartate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartate racemase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | depletion of aspartate racemase by retrovirus-mediated expression of short-hairpin RNA in newborn neurons of the adult hippocampus elicits profound defects in the dendritic development and survival of newborn neurons and survival | Mus musculus |
| physiological function | aspartate racemase regulates adult neurogenesis | Mus musculus |
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Release 2023.1 (January 2023)
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