Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | 10 mM ADP increases the activity to 320% of the control | Anadara broughtonii | |
AMP | 10 mM AMP increases the activity to 399% of the control | Anadara broughtonii |
Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Anadara broughtonii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the cDNA included an open reading frame of 1017 bp encoding a protein of 338 amino acids, and the deduced amino acid sequence contains a pyridoxal 5'-phosphate-binding motif | Anadara broughtonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 10 mM ATP decreases the activity to 23% | Anadara broughtonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.5 | - |
L-aspartate | - |
Anadara broughtonii | |
17.2 | - |
D-Aspartate | - |
Anadara broughtonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37100 | - |
predicted from cDNA | Anadara broughtonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anadara broughtonii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
aspartate racemase is purified from the Scapharca broughtonii foot muscle by a procedure that includes sodium sulfate fractionation and blue sepharose column chromatography. The purified enzyme (225 mg) is digested at 25°C for 12 h with 80 mg of lysyl endopeptidase in a reaction mixture comprising 50 mM Tris-HCl, pH 8.0, containing 2 M guanidine hydrochloride, and the resulting peptides is resolved by reverse phase high performance liquid chromatography on a capcell Pac C-8 column (0.2 mm x 15 cm) using an acetonitrile gradient in 0.06% trifluoroacetic acid | Anadara broughtonii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate = D-aspartate | the enzyme racemizes only aspartate and no other amino acids but the enzyme also shows a dehydratase activity toward L-threo-3-hydroxyaspartate | Anadara broughtonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
foot muscle | enzyme is purified from foot muscle of Scapharca broughtonii | Anadara broughtonii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Anadara broughtonii | D-aspartate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Aspartate racemase | - |
Anadara broughtonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Anadara broughtonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Anadara broughtonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | this enzyme is the first aspartate racemase purified from animal tissues and unique in its pyridoxal 5'-phosphate (PLP)dependence in contrast to microbial aspartate racemases characterized so far | Anadara broughtonii |