KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
D-Lys | - |
Pseudomonas putida | |
0.55 | - |
L-Lys | - |
Pseudomonas putida | |
9.8 | - |
D-Thr | - |
Pseudomonas putida | |
31 | - |
L-Thr | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Pseudomonas putida |
82000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
920 | - |
L-Lys as substrate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arg | 80% of the activity relative to L-Lys | Pseudomonas putida | L-Arg | - |
? | |
D-Thr | - |
Pseudomonas putida | L-Thr | - |
r | |
L-Ala | 6% of the activity relative to L-Lys | Pseudomonas putida | D-Ala | - |
? | |
L-Asn | 12% of the activity relative to L-Lys | Pseudomonas putida | D-Asn | - |
? | |
L-Citrulline | 45% of the activity relative to L-Lys | Pseudomonas putida | D-Citrulline | - |
? | |
L-Ethionine | 83% of the activity relative to L-Lys | Pseudomonas putida | D-Ethionine | - |
? | |
L-Glu | 80% of the activity relative to L-Lys | Pseudomonas putida | D-Glu | - |
? | |
L-His | 6% of the activity relative to L-Lys | Pseudomonas putida | D-His | - |
? | |
L-Homoarginine | 80% of the activity relative to L-Lys | Pseudomonas putida | L-Homoarginine | - |
? | |
L-Homocitrulline | 45% of the activity relative to L-Lys | Pseudomonas putida | D-Homocitrulline | - |
? | |
L-homoserine | 22% of the activity relative to L-Lys | Pseudomonas putida | D-homoserine | - |
? | |
L-Leu | 10% of the activity relative to L-Lys | Pseudomonas putida | D-Leu | - |
? | |
L-Lys | - |
Pseudomonas putida | D-Lys | - |
? | |
L-Met | 66% of the activity relative to L-Lys | Pseudomonas putida | D-Met | - |
? | |
L-Norleucine | 45% of the activity relative to L-Lys | Pseudomonas putida | D-Norleucine | - |
? | |
L-Orn | 80% of the activity relative to L-Lys | Pseudomonas putida | D-Orn | - |
? | |
L-Ser | 10% of the activity relative to L-Lys | Pseudomonas putida | D-Ser | - |
? | |
L-Thr | 3% of the activity relative to L-Lys | Pseudomonas putida | L-Thr | - |
? | |
N6-Acetyl-L-Lys | 80% of the activity relative to L-Lys | Pseudomonas putida | N6-Acetyl-D-Lys | - |
? | |
S-Methyl-L-Cys | 3% of the activity relative to L-Lys | Pseudomonas putida | S-Methyl-D-Cys | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme | Pseudomonas putida | |
pyridoxal 5'-phosphate | contains 1 mol of pyridoxal 5'-phosphate per mol of subunit | Pseudomonas putida |