Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.10 extracted from

  • Inagaki, K.; Tanizawa, K.; Tanaka, H.; Soda, K.
    Purification and characterization of amino acid racemase with very broad substrate specificity from Aeromonas caviae (1987), Agric. Biol. Chem., 51, 173-180.
No PubMed abstract available
Search for more literature for 5.1.1.10

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
 Aeromonas caviae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.83
-
 D-Lys
-
 Aeromonas caviae
0.9
-
 L-Orn
-
 Aeromonas caviae
1
-
 D-Ser
-
 Aeromonas caviae
1.05
-
 L-Lys
-
 Aeromonas caviae
1.2
-
 L-Met
-
 Aeromonas caviae
1.6
-
 D-Met
-
 Aeromonas caviae
2.5
-
 L-Lys D-Lys Aeromonas caviae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Aeromonas caviae
73000
-
 gel filtration Aeromonas caviae
76000
-
 sedimentation equilibrium method Aeromonas caviae

Organism

Organism UniProt Comment Textmining
Aeromonas caviae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas caviae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1250
-
 L-Lys as substrate Aeromonas caviae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arg 65% of the activity relative to L-Lys Aeromonas caviae L-Arg
-
 ?
D-Ser
-
 Aeromonas caviae L-Ser
-
 r
L-Asn 9% of the activity relative to L-Lys Aeromonas caviae D-Asn
-
 ?
L-Citrulline 30% of the activity relative to L-Lys Aeromonas caviae D-Citrulline
-
 ?
L-Ethionine 67% of the activity relative to L-Lys Aeromonas caviae D-Ethionine
-
 ?
L-Glu 57% of the activity relative to L-Lys Aeromonas caviae D-Glu
-
 ?
L-Homoarginine 67% of the activity relative to L-Lys Aeromonas caviae L-Homoarginine
-
 ?
L-Homocitrulline 34% of the activity relative to L-Lys Aeromonas caviae D-Homocitrulline
-
 ?
L-Homocysteine 55% of the activity relative to L-Lys Aeromonas caviae D-Homocysteine
-
 ?
L-homoserine 12% of the activity relative to L-Lys Aeromonas caviae D-homoserine
-
 ?
L-Leu 12% of the activity relative to L-Lys Aeromonas caviae D-Leu
-
 ?
L-Lys
-
 Aeromonas caviae D-Lys
-
 ?
L-Met 67% of the activity relative to L-Lys Aeromonas caviae D-Met
-
 ?
L-Norleucine 28% of the activity relative to L-Lys Aeromonas caviae D-Norleucine
-
 ?
L-Orn 79% of the activity relative to L-Lys Aeromonas caviae D-Orn
-
 ?
L-Selenohomocysteine 27% of the activity relative to L-Lys Aeromonas caviae D-Selenohomocysteine
-
 ?
N6-Acetyl-L-Lys 37% of the activity relative to L-Lys Aeromonas caviae N6-Acetyl-D-Lys
-
 ?
S-Methyl-L-Cys 43% of the activity relative to L-Lys Aeromonas caviae S-Methyl-D-Cys
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Aeromonas caviae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aeromonas caviae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to 60°C, pH 8.0, 10 min, in 0.1 M potassium phosphate Aeromonas caviae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
 Aeromonas caviae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Aeromonas caviae
pyridoxal 5'-phosphate required for maximal activity Aeromonas caviae
pyridoxal 5'-phosphate Km: 0.0026 mM Aeromonas caviae