Cloned (Comment) | Organism |
---|---|
gene alr-2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aeromonas hydrophila subsp. hydrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(9-acridinylamino)benzenepropanoic acid | - |
Aeromonas hydrophila subsp. hydrophila | |
anabellamide | - |
Aeromonas hydrophila subsp. hydrophila | |
D-cycloserine | - |
Aeromonas hydrophila subsp. hydrophila | |
hematoxylin | - |
Aeromonas hydrophila subsp. hydrophila | |
higenamine | - |
Aeromonas hydrophila subsp. hydrophila | |
homogentisic acid | competitive inhibition, has minimal cytotoxicity against mammalian cells. Homogentisic acid binds to the active site of the racemase | Aeromonas hydrophila subsp. hydrophila | |
hydroquinone | noncompetitive inhibition, has minimal cytotoxicity against mammalian cells. Hydroquinone binds near the active center of alanine racemase | Aeromonas hydrophila subsp. hydrophila | |
additional information | inhibitor screening of a library of 2100 compounds, and molecular docking study | Aeromonas hydrophila subsp. hydrophila | |
patulin | - |
Aeromonas hydrophila subsp. hydrophila | |
propyl gallate | - |
Aeromonas hydrophila subsp. hydrophila | |
quercetin | - |
Aeromonas hydrophila subsp. hydrophila | |
[4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid | - |
Aeromonas hydrophila subsp. hydrophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Aeromonas hydrophila subsp. hydrophila | - |
D-alanine | - |
r | |
L-alanine | Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila subsp. hydrophila | A0KL98 | - |
- |
Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 | A0KL98 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Aeromonas hydrophila subsp. hydrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Aeromonas hydrophila subsp. hydrophila | D-alanine | - |
r | |
L-alanine | - |
Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 | D-alanine | - |
r | |
additional information | assay method optimization and validation, overview | Aeromonas hydrophila subsp. hydrophila | ? | - |
? | |
additional information | assay method optimization and validation, overview | Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Aeromonas hydrophila subsp. hydrophila |
Synonyms | Comment | Organism |
---|---|---|
alr-2 | - |
Aeromonas hydrophila subsp. hydrophila |
alr-2 | gen ename | Aeromonas hydrophila subsp. hydrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Aeromonas hydrophila subsp. hydrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aeromonas hydrophila subsp. hydrophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Aeromonas hydrophila subsp. hydrophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0517 | - |
homogentisic acid | pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | |
0.212 | - |
hydroquinone | pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0054 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | D-cycloserine | |
0.0066 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | anabellamide | |
0.0086 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | propyl gallate | |
0.0089 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | [4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid | |
0.0125 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | homogentisic acid | |
0.0143 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | higenamine | |
0.0147 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | patulin | |
0.0155 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | quercetin | |
0.0156 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | hematoxylin | |
0.0177 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | 2-(9-acridinylamino)benzenepropanoic acid | |
0.0185 | - |
pH 11.0, 35°C, recombinant His-tagged enzyme | Aeromonas hydrophila subsp. hydrophila | hydroquinone |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology modeling using the crystal structure of Escherichia coli AlaR with PDB ID 2rjh | Aeromonas hydrophila subsp. hydrophila |
physiological function | the enzyme catalyzes the interconversion of L-alanine to D-alanine. D-Alanine is an essential building block of the cell wall of both gram-positive and gram-negative bacteria | Aeromonas hydrophila subsp. hydrophila |