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Literature summary for 5.1.1.1 extracted from

  • Kato, S.; Hemmi, H.; Yoshimura, T.
    Lysine racemase from a lactic acid bacterium, Oenococcus oeni: structural basis of substrate specificity (2012), J. Biochem., 152, 505-508.
    View publication on PubMed
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene alr, expression of the enzyme in Escherichia coli strain BL21 Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
I222T site-directed mutagenesis, the mutant is an alanine racemase with lysine racemization activity Geobacillus stearothermophilus
I222T/Y354W site-directed mutagenesis, the double mutant is an alanine racemase with lysine racemization activity Geobacillus stearothermophilus
Y354W site-directed mutagenesis, the mutant is an alanine racemase with lysine racemization activity Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
 D-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
2.6
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus
2.8
-
 D-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus
4
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
4.5
-
 L-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
4.7
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus
5.1
-
 L-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus
8.3
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
18
-
 D-Lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
32
-
 L-lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Geobacillus stearothermophilus
-
 D-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P10724
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine
-
 Geobacillus stearothermophilus L-alanine
-
 r
D-lysine enzyme mutant I222T/Y354W, no activity with the wild-type enzyme Geobacillus stearothermophilus L-lysine
-
 ?
L-alanine
-
 Geobacillus stearothermophilus D-alanine
-
 r
L-lysine enzyme mutant I222T/Y354W, no activity with the wild-type enzyme Geobacillus stearothermophilus D-lysine
-
 ?
additional information residues Ile222 and Tyr354 are important for the enzyme substrate specificity Geobacillus stearothermophilus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 D-Lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
4.17
-
 L-lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
517
-
 D-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
633
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
983
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus
1017
-
 D-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus
1133
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
1133
-
 L-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
1417
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus
1533
-
 L-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 L-lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
0.14
-
 D-Lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
136.7
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
158.3
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W Geobacillus stearothermophilus
216.7
-
 D-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
250
-
 L-alanine pH 8.0, 30°C, recombinant mutant Y354W Geobacillus stearothermophilus
300
-
 L-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus
300
-
 L-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus
366.7
-
 D-alanine pH 8.0, 30°C, recombinant wild-type enzyme Geobacillus stearothermophilus
383.3
-
 D-alanine pH 8.0, 30°C, recombinant mutant I222T Geobacillus stearothermophilus