Application | Comment | Organism |
---|---|---|
drug development | the enzyme is an attractive target for antimicrobial drug development | Acinetobacter baumannii |
Cloned (Comment) | Organism |
---|---|
gene alr from strain OXA-23, DNA and amino acid sequence determination and analysis, expression of recombinant N-terminally His7-tagged enzyme in Escherichia coli strain BL21(DE3) | Acinetobacter baumannii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 9 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 15 mM NaCl, 10% v/v glycerol, 3 mM 2-mercaptoethanol, and 0.1 mM pyridoxal-5'-phosphate, with 0.001 ml of reservoir solution containing 2% v/v Tacsimate pH 5.0, 0.1 M sodium citrate tribasic dihydrate, pH 5.5, 16% w/v PEG 3350, equilibration against 1 ml of reservoir solution, method optimization, 2 days, X-ray diffraction structure determination and analysis at 2.3 A resolution | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant His7-tagged enzyme, SDS-PAGE | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Acinetobacter baumannii | - |
D-alanine | - |
r | |
L-alanine | Acinetobacter baumannii OXA-23 | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
isolated from a urine specimen of a patient hospitalized in Busan, Republic of Korea, gene alr | - |
Acinetobacter baumannii OXA-23 | - |
isolated from a urine specimen of a patient hospitalized in Busan, Republic of Korea, gene alr | - |
Purification (Comment) | Organism |
---|---|
recombinant His7-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatographyy | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Acinetobacter baumannii | D-alanine | - |
r | |
L-alanine | - |
Acinetobacter baumannii OXA-23 | D-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant His7-tagged enzyme, SDS-PAGE | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
ALR | - |
Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
physiological function | alanine racemase catalyzes the racemization of L-alanine to D-alanine, which is a key component of the peptidoglycan layer, especially in cross-linking the bacterial cell walls | Acinetobacter baumannii |