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Literature summary for 5.1.1.1 extracted from

  • Nguyen, D.D.; Ngo, H.P.; Hong, M.K.; Pham, T.V.; Lee, J.H.; Lee, J.J.; Kwon, D.B.; Lee, S.H.; Kang, L.W.
    Expression, crystallization and preliminary X-ray crystallographic analysis of alanine racemase from Acinetobacter baumannii OXA-23 (2013), Acta Crystallogr. Sect. F, 69, 1041-1044.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is an attractive target for antimicrobial drug development Acinetobacter baumannii

Cloned(Commentary)

Cloned (Comment) Organism
gene alr from strain OXA-23, DNA and amino acid sequence determination and analysis, expression of recombinant N-terminally His7-tagged enzyme in Escherichia coli strain BL21(DE3) Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 9 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 15 mM NaCl, 10% v/v glycerol, 3 mM 2-mercaptoethanol, and 0.1 mM pyridoxal-5'-phosphate, with 0.001 ml of reservoir solution containing 2% v/v Tacsimate pH 5.0, 0.1 M sodium citrate tribasic dihydrate, pH 5.5, 16% w/v PEG 3350, equilibration against 1 ml of reservoir solution, method optimization, 2 days, X-ray diffraction structure determination and analysis at 2.3 A resolution Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, recombinant His7-tagged enzyme, SDS-PAGE Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Acinetobacter baumannii
-
D-alanine
-
r
L-alanine Acinetobacter baumannii OXA-23
-
D-alanine
-
r

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
isolated from a urine specimen of a patient hospitalized in Busan, Republic of Korea, gene alr
-
Acinetobacter baumannii OXA-23
-
isolated from a urine specimen of a patient hospitalized in Busan, Republic of Korea, gene alr
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His7-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatographyy Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
Acinetobacter baumannii D-alanine
-
r
L-alanine
-
Acinetobacter baumannii OXA-23 D-alanine
-
r

Subunits

Subunits Comment Organism
? x * 42000, recombinant His7-tagged enzyme, SDS-PAGE Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
ALR
-
Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Acinetobacter baumannii

General Information

General Information Comment Organism
physiological function alanine racemase catalyzes the racemization of L-alanine to D-alanine, which is a key component of the peptidoglycan layer, especially in cross-linking the bacterial cell walls Acinetobacter baumannii