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Literature summary for 5.1.1.1 extracted from
- Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50) (2012), Acta Crystallogr. Sect. D, 68, 82-92.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme expression in Escherichia coli strain BL21(DE3)/pMB1978 | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme from strain Mu50, sitting drop method, mixing of 12 mg/ml protein in 20 mM Tris-HCl, pH 7.6, with 100 mM sodium acetate trihydrate, pH 5.0, and 1.8 M ammonium sulfate, 7 days, X-ray diffraction structure determination and analysis at 2.15 A resolution, molecular replacement | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the active-site binding pocket, dimer interface and active-site entryway of the enzyme are potential targets for structure-aided inhibitor design, formation of a template for structure-based drug-development efforts targeting the enzyme, overview | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.89 | - |
D-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus |  |
| 2.77 | - |
L-alanine | pH not specified in the publication, 30°C, recombinant enzyme | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42800 | - |
2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
| 73000 | - |
recombinant enzyme, dynamic light scattering | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine | Staphylococcus aureus | - |
D-alanine | - |
r | |
| L-alanine | Staphylococcus aureus Mu50 | - |
D-alanine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | P63479 | - |
- |
| Staphylococcus aureus Mu50 | P63479 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3)/pMB1978 by anion exchange and hydrophobic interaction chromatography, followed by gel filtration and dialysis to over 95% purity | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine | - |
Staphylococcus aureus | D-alanine | - |
r | |
| L-alanine | - |
Staphylococcus aureus Mu50 | D-alanine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42800, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
| More | dimer interfaces for the enzyme is 2510 A2 | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALR | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Staphylococcus aureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | because D-alanine is an essential component of the bacterial cell-wall peptidoglycan, inhibition of alanine racemase is lethal to prokaryotes | Staphylococcus aureus |
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