Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.1 extracted from

  • Yoshimura, T.; Goto, M.
    D-amino acids in the brain: structure and function of pyridoxal phosphate-dependent amino acid racemases (2008), FEBS J., 275, 3527-3537.
    View publication on PubMed
Search for more literature for 5.1.1.1

Protein Variants

Protein Variants Comment Organism
K39A Lys39 is the catalytic residue required for the abstraction and addition of the alpha-hydrogen of D-alanine, As shown by site-directed mutagenesis (K39A mutant) and chemical rescue studies. Tyr265 is catalytic residue for L-alanine, shown by site-directed mutagenesis (Y265A mutant) Geobacillus stearothermophilus
Y265A Lys39 is the catalytic residue required for the abstraction and addition of the alpha-hydrogen of D-alanine, As shown by site-directed mutagenesis (K39A mutant) and chemical rescue studies. Tyr265 is catalytic residue for L-alanine, shown by site-directed mutagenesis (Y265A mutant) Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine Salmonella enterica subsp. enterica serovar Typhimurium
-
 D-alanine
-
 ?
L-alanine Bacillus subtilis
-
 D-alanine
-
 ?
L-alanine Escherichia coli
-
 D-alanine
-
 ?
L-alanine Geobacillus stearothermophilus
-
 D-alanine
-
 ?
L-alanine Bipolaris zeicola
-
 D-alanine responsible for the synthesis of the d-alanine moiety present in cyclosporin A and of HC-toxin ?
L-alanine Tolypocladium inflatum responsible for the synthesis of the d-alanine moiety present in cyclosporin A and of HC-toxin D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bipolaris zeicola
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus P10724
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Tolypocladium inflatum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-alanine
-
 ?
L-alanine
-
 Bacillus subtilis D-alanine
-
 ?
L-alanine
-
 Escherichia coli D-alanine
-
 ?
L-alanine
-
 Geobacillus stearothermophilus D-alanine
-
 ?
L-alanine
-
 Bipolaris zeicola D-alanine responsible for the synthesis of the d-alanine moiety present in cyclosporin A and of HC-toxin ?
L-alanine responsible for the synthesis of the d-alanine moiety present in cyclosporin A and of HC-toxin Tolypocladium inflatum D-alanine
-
 ?

Synonyms

Synonyms Comment Organism
ALR
-
 Geobacillus stearothermophilus
DadB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
DadB
-
 Bacillus subtilis
DadB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Tolypocladium inflatum
pyridoxal 5'-phosphate
-
 Bipolaris zeicola
pyridoxal 5'-phosphate once per 1 million turnovers of racemization a H-atom is added to C4-atom of the substrate moiety of the anionic intermediate instead of the reprotonation of the abstracted hydrogen at C(alpha) resulting in pyridoxamine 5'-phosphate Geobacillus stearothermophilus
pyridoxal 5'-phosphate stabilizes anionic intermediate after abstraction of alpha-hydrogen of the substrate amino acid by forming a quinoid intermediate Salmonella enterica subsp. enterica serovar Typhimurium
pyridoxal 5'-phosphate stabilizes anionic intermediate after abstraction of alpha-hydrogen of the substrate amino acid by forming a quinoid intermediate Bacillus subtilis
pyridoxal 5'-phosphate stabilizes anionic intermediate after abstraction of alpha-hydrogen of the substrate amino acid by forming a quinoid intermediate Escherichia coli