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Literature summary for 5.1.1.1 extracted from

  • Yokoyama, T.; Mimura, Y.; Sato, M.; Nagahisa, E.
    Purification and some properties of alanine racemase from the marine gastropod Cellana grata (2006), Fish. Sci., 72, 195-201.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
aminooxyacetic acid
-
Cellana grata
hydroxylamine
-
Cellana grata
phenylhydrazine
-
Cellana grata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Vmax 110 micromol/min/mg D-alanine Cellana grata
additional information
-
additional information Vmax 323 micromol/min/mg L-alanine Cellana grata
20.4
-
D-alanine HPLC analysis Cellana grata
43
-
L-alanine HPLC analysis Cellana grata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
SDS-PAGE Cellana grata
41400
-
gel filtration Cellana grata

Organism

Organism UniProt Comment Textmining
Cellana grata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
alanine racemase is purified from the muscle of C. grata by using Toyopearl DEAE, Toyopearl Butyl, Hydroxyapatite and Sephacryl S200 column chromatographies. Cellana grata

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Cellana grata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
288 units/mg Cellana grata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine
-
Cellana grata L-alanine
-
r
L-alanine
-
Cellana grata D-alanine
-
r

Subunits

Subunits Comment Organism
monomer 1 * 40500, SDS-PAGE Cellana grata

Synonyms

Synonyms Comment Organism
alanine racemase
-
Cellana grata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Cellana grata

Cofactor

Cofactor Comment Organism Structure
additional information not activated by pyridoxal 5'-phosphate Cellana grata

pI Value

Organism Comment pI Value Maximum pI Value
Cellana grata isoelectric focusing
-
5.2