Cloned (Comment) | Organism |
---|---|
amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products | Mycobacterium avium |
amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloserine | D-cycloserine or a racemic mixture of D- and L-cycloserine | Mycobacterium avium | |
cycloserine | D-cycloserine or a racemic mixture of D- and L-cycloserine | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-Ala | - |
Mycobacterium avium | |
0.5 | - |
L-Ala | - |
Mycobacterium avium | |
1.1 | - |
D-Ala | 23°C | Mycobacterium tuberculosis | |
1.2 | - |
L-Ala | 23°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium avium | - |
- |
- |
Mycobacterium tuberculosis | P9WQA9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQA9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium avium |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Mycobacterium avium | D-Ala | - |
r | |
L-Ala | - |
Mycobacterium tuberculosis | D-Ala | - |
r | |
L-Ala | - |
Mycobacterium tuberculosis H37Rv | D-Ala | - |
r |