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Literature summary for 5.1.1.1 extracted from
- Purification and properties of alanine racemase from crayfish Procambarus clarkii (2000), Comp. Biochem. Physiol. B, 126, 599-608.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminooxyacetic acid | 1 mM, complete inhibition | Procambarus clarkii |  |
| D-cycloserine | 1 mM, 95% inhibition | Procambarus clarkii | |
| hydroxylamine | 1 mM, complete inhibition | Procambarus clarkii | |
| L-Cycloserine | 1 mM, 90% inhibition | Procambarus clarkii | |
| NaCl | at concentrations around seawater level | Procambarus clarkii | |
| NEM | 1 mM, 22% inhibition | Procambarus clarkii | |
| PCMB | 1 mM, 91% inhibition | Procambarus clarkii | |
| phenylhydrazine | 1 mM, 72% inhibition | Procambarus clarkii | |
| pyridoxal 5'-phosphate | 0.4 mM, 51% inhibition | Procambarus clarkii | |
| Sodium borohydride | 1 mM, 30% inhibition | Procambarus clarkii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effect of NaCl on Km-value | Procambarus clarkii | |
| 73.5 | - |
D-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
| 171 | - |
L-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000, SDS-PAGE | Procambarus clarkii |
| 60000 | - |
gel filtration | Procambarus clarkii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Procambarus clarkii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Procambarus clarkii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2044 | - |
- |
Procambarus clarkii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM potassium phosphate buffer, pH 7.2, 0.01 mM pyridoxal 5'-phosphate, 6 months, 20% loss of activity | Procambarus clarkii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala | the ratio of the activity for conversion of D-alanine to L-alanine to that of the reverse conversion is constantly about 0.5 in the pH range 7-9.5 | Procambarus clarkii | D-Ala | - |
r | |
| additional information | no racemization of L-Ser, L-Asp, L-Glu, L-Val and L-Arg | Procambarus clarkii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Procambarus clarkii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Procambarus clarkii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3270 | - |
D-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
| 3272 | - |
D-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
| 7500 | - |
L-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
| 7504 | - |
L-Ala | 30°C, pH 8.5 | Procambarus clarkii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
D-Ala as substrate | Procambarus clarkii |
| 9 | - |
L-Ala as substrate | Procambarus clarkii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | enzyme is dependent on, maximal activity at 0.025 mM | Procambarus clarkii | |
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