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Literature summary for 5.1.1.1 extracted from
- Alanine racemase from an acidophile, Acidiphilium organovorum: purification and characterization (1998), Biosci. Biotechnol. Biochem., 62, 242-247.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-penicillamine | 1 mM, 79% inhibition | Acidiphilium organovorum |  |
| hydroxylamine | 1 mM, 68% inhibition | Acidiphilium organovorum | |
| L-Penicillamine | 1 mM, 28% inhibition | Acidiphilium organovorum | |
| NaBH4 | 0.5 M, loss of activity | Acidiphilium organovorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
D-Ala | pH 9.0 | Acidiphilium organovorum | |
| 7.9 | - |
L-Ala | pH 9.0 | Acidiphilium organovorum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Acidiphilium organovorum | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Acidiphilium organovorum |
| 65000 | - |
gel filtration | Acidiphilium organovorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidiphilium organovorum | - |
13H | - |
| Acidiphilium organovorum 13H | - |
13H | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidiphilium organovorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 191 | - |
- |
Acidiphilium organovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala | highly specific for Ala | Acidiphilium organovorum | D-Ala | - |
r | |
| L-Ala | highly specific for Ala | Acidiphilium organovorum 13H | D-Ala | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Acidiphilium organovorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Acidiphilium organovorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
30 min, no loss of activity | Acidiphilium organovorum |
| 70 | - |
30 min, enzyme loses most of its activity | Acidiphilium organovorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Acidiphilium organovorum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1 mol of enzyme contains 2 mol of cofactor | Acidiphilium organovorum | |
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