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Literature summary for 5.1.1.1 extracted from

  • Okubo, Y.; Tomioka, R.; Yokoigawa, K.; Kawai, H.
    Lability of alanine racemase from a psychrotroph (1995), J. Home Econ. Jpn., 46, 1135-1140.
No PubMed abstract available
Search for more literature for 5.1.1.1

General Stability

General Stability Organism
5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Pseudomonas fluorescens
5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Geobacillus stearothermophilus
denatured by 3.5 M urea in one transition phase Geobacillus stearothermophilus
denatured by urea in two transition phases, dissociation of pyridoxal 5'-phosphate with 4.0 M urea, unfolding with 5.5 M urea Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values of L-Ala in the presence of urea at various concentrations Geobacillus stearothermophilus
additional information
-
 additional information Km values of L-Ala in the presence of urea at various concentrations Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala
-
 Geobacillus stearothermophilus D-Ala
-
 ?
L-Ala
-
 Pseudomonas fluorescens D-Ala
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 over 30°C, 1 h, quick loss of activity Pseudomonas fluorescens
30
-
 5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Pseudomonas fluorescens
30
-
 5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Geobacillus stearothermophilus
75
-
 1 h, inactivation over 75°C Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.3 10.5 1 h, 0°C, stable Geobacillus stearothermophilus
8.3 10.5 1 h, 0°C, stable Pseudomonas fluorescens