Protein Variants | Comment | Organism |
---|---|---|
additional information | double mutant for the alr encoded enzyme and the dad B encoded enzyme display a phenotype of requirement for exogenous D-Ala for growth | Salmonella enterica subsp. enterica serovar Typhimurium |
additional information | mutant gene which tandemly encodes the two polypeptides of the enzyme subunit, fragment 1 and fragment 2, cleaved at the position corresponding to the predicted hinge region. The mutant fragmentary alanine racemase is active at about 40% of the activity of the wild type enzyme | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | alr racemase is constitutive and serves an anabolic function, dadB encoded enzyme is inducible and required for cell growth on L-Ala | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Enterococcus faecalis | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Salmonella enterica subsp. enterica serovar Typhimurium | D-Ala | - |
? | |
L-Ala | - |
Bacillus subtilis | D-Ala | - |
? | |
L-Ala | - |
Geobacillus stearothermophilus | D-Ala | - |
? | |
L-Ala | - |
Pseudomonas putida | D-Ala | - |
? | |
L-Ala | - |
Enterococcus faecalis | D-Ala | - |
? | |
additional information | alr racemase is constitutive and serves an anabolic function, dadB encoded enzyme is inducible and required for cell growth on L-Ala | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Geobacillus stearothermophilus |
monomer | - |
Enterococcus faecalis |
monomer | dadB enzyme and alr enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
80 min, pH 7.2, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
80°C, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate binds to Lys of the enzyme protein and forms an aldimine Schiff base. The alpha-proton of the substrate is then abstracted, and the pyridoxal 5'-phosphate carbanion is generated | Salmonella enterica subsp. enterica serovar Typhimurium | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate binds to Lys of the enzyme protein and forms an aldimine Schiff base. The alpha-proton of the substrate is then abstracted, and the pyridoxal 5'-phosphate carbanion is generated | Bacillus subtilis | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate binds to Lys of the enzyme protein and forms an aldimine Schiff base. The alpha-proton of the substrate is then abstracted, and the pyridoxal 5'-phosphate carbanion is generated | Geobacillus stearothermophilus | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate binds to Lys of the enzyme protein and forms an aldimine Schiff base. The alpha-proton of the substrate is then abstracted, and the pyridoxal 5'-phosphate carbanion is generated | Enterococcus faecalis | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent enzyme | Bacillus subtilis | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent enzyme | Geobacillus stearothermophilus | |
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent enzyme | Enterococcus faecalis |