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Literature summary for 5.1.1.1 extracted from

  • Wasserman, S.A.; Daub, E.; Grisafi, P.; Botstein, D.; Walsh, C.T.
    Catabolic alanine racemase from Salmonella typhimurium: DNA sequence, enzyme purification, and characterization (1984), Biochemistry, 23, 5182-5187.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
dadB Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability Organism
stable in 30% ammonium sulfate. Irreversibly diminished activity by exposure to ammonium sulfate concentrations near or above 40%, where precipitation occurs Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 D-Ala
-
 Salmonella enterica subsp. enterica serovar Typhimurium
8.2
-
 L-Ala
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 1 * 39000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
39044
-
 1 * 39044, calculation from nucleotide sequence Salmonella enterica subsp. enterica serovar Typhimurium
50000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme encoded by the dadB gene Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
910
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
-70°C, 10% glycerol, more than 90% of the activity is retained after 2 years Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-Ala
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39044, calculation from nucleotide sequence Salmonella enterica subsp. enterica serovar Typhimurium
monomer 1 * 39000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 mol of pyridoxal 5'-phosphate is bound per subunit Salmonella enterica subsp. enterica serovar Typhimurium