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Literature summary for 4.99.1.9 extracted from
- Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis (1994), Eur. J. Biochem., 220, 201-208.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloned in pUC18, expression in Escherichia coli JM109/pLUGlSe2 | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | 2 mM, 99% inactivation after incubation for 2 h. Protoporphyrin IX or Zn2+ at concentrations of 0.8 mM and 20 mM, respectively, does not protect | Bacillus subtilis |  |
| p-chloromercuriphenylsulfonate | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
protoporphyrin IX | pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction | Bacillus subtilis | |
| 0.017 | - |
Zn2+ | pH 7.2, 30°C | Bacillus subtilis | |
| 0.17 | - |
Cu2+ | pH 7.2, 30°C | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35348 | - |
1 * 35348, calculated from sequence | Bacillus subtilis |
| 40000 | - |
gel filtration | Bacillus subtilis |
| 40500 | - |
1 * 40500, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, the purified enzyme is stable for at least five months | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin IX + Cu2+ | - |
Bacillus subtilis | Cu-protoporphyrin IX + 2 H+ | - |
? | |
| protoporphyrin IX + Fe2+ | the enzyme shows no detectable activity with Ca2+, Co2+, Fe3+, Mg2+, or Rb+ | Bacillus subtilis | Fe-protoporphyrin IX + 2 H+ | - |
? | |
| protoporphyrin IX + Zn2+ | - |
Bacillus subtilis | Zn-protoporphyrin IX + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35348, calculated from sequence | Bacillus subtilis |
| monomer | 1 * 40500, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hemH | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
protoporphyrin IX | pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Bacillus subtilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme participates in heme biosynthesis | Bacillus subtilis |
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