Literature summary for 4.99.1.8 extracted from

Nakatani, K.; Ishikawa, H.; Aono, S.; Mizutani, Y.
Identification of essential histidine residues involved in heme binding and hemozoin formation in heme detoxification protein from Plasmodium falciparum (2014), Sci. Rep., 4, 6137.

Search for more literature for 4.99.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
H122A	the mutant shows less than half of the wild type activity	Plasmodium falciparum
H172A	the mutant shows 44% of the wild type activity	Plasmodium falciparum
H175A	the mutant shows less than half of the wild type activity	Plasmodium falciparum
H192A	the mutant shows decreased hemozoin formation activity compared to the wild type enzyme	Plasmodium falciparum
H197A	the mutant shows less than half of the wild type activity	Plasmodium falciparum
H44A	the mutant shows decreased hemozoin formation activity compared to the wild type enzyme	Plasmodium falciparum
H44A/H58A/H70A/H79A/H122A/H172A/H175A/H192A/H197A	the mutant shows less than half of the wild type activity	Plasmodium falciparum
H58A	the mutant shows decreased hemozoin formation activity compared to the wild type enzyme	Plasmodium falciparum
H70A	the mutant shows decreased hemozoin formation activity compared to the wild type enzyme	Plasmodium falciparum
H79A	the mutant shows decreased hemozoin formation activity compared to the wild type enzyme	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q8IL04	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q column chromatography and Sephadex G25 gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
heme	-	Plasmodium falciparum	hemozoin	-	?

Synonyms

Synonyms	Comment	Organism
HDP	-	Plasmodium falciparum
heme detoxification protein	-	Plasmodium falciparum

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Release 2023.1 (January 2023)