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Literature summary for 4.99.1.8 extracted from

  • Jani, D.; Nagakatti, R.; Beatty, W.; Angel, R.; Slebodnick, C.; Andersen, J.; Kumar, S.; Rathore, D.
    HDP - a novel heme detoxification protein from the malaria parasite (2008), PLos Pathog., 4, e1000053.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.99.1.8

Application

Application Comment Organism
medicine involvement of heme detoxification protein in the process of formation of hemozoin suggests that it could be a malaria drug target Plasmodium falciparum
medicine involvement of heme detoxification protein in the process of formation of hemozoin suggests that it could be a malaria drug target Plasmodium yoelii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium yoelii
expression of full length enzyme in Escherichia coli, expression of two truncated enzyme proteins (HDP3 encoded by amino acids 88-205 of the full-length protein, representing the fasciclin domain and HDP2 encoded by amino acids 1–87 and lacking the fasciclin domain) Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information the truncated enzyme proteins HDP3 (encoded by amino acids 88-205 of the full-length protein, representing the fasciclin domain) and HDP2 (encoded by amino acids 1–87 and lacking the fasciclin domain) are unable to produce hemozoin. The full-length enzyme is required for heme binding and hemozoin production activities of the protein Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the parasite utilizes a circuitous outbound-inbound trafficking route by initially secreting HDP into the cytosol of infected red blood cells Plasmodium falciparum
-
-
food vacuole
-
 Plasmodium falciparum 20020
-

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium yoelii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, full-length recombinant enzyme and truncated enzymes Plasmodium falciparum
recombinant enzyme Plasmodium yoelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferriprotoporphyrin IX
-
 Plasmodium falciparum beta-hematin
-
 ?
ferriprotoporphyrin IX HDP possesses 2.7 heme binding sites Plasmodium yoelii beta-hematin
-
 ?

Synonyms

Synonyms Comment Organism
HDP
-
 Plasmodium falciparum
HDP
-
 Plasmodium yoelii
heme detoxification protein
-
 Plasmodium falciparum
heme detoxification protein
-
 Plasmodium yoelii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
94
-
 10 min, stable Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4.4
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
3.3 5.2 pH 3.3-4.4: optimum, pH 5.2: about 75% of maximal activity, no activity above pH 5.6 Plasmodium falciparum