Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 codonplus (RIPL) cells | Paracoccus pantotrophus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using in 0.2 M imidazole malate pH 5.5, 24% (w/v) PEG 600 at a ratio of 50:50 ratio of protein : mother liquor and 10% ethylene glycol | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Paracoccus pantotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Paracoccus pantotrophus | - |
siroheme + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chelating Sepharose column chromatography | Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | - |
Paracoccus pantotrophus | siroheme + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Paracoccus pantotrophus |
Synonyms | Comment | Organism |
---|---|---|
CbiX | - |
Paracoccus pantotrophus |
CysG | - |
Paracoccus pantotrophus |
Met8p | - |
Paracoccus pantotrophus |
SirB | - |
Paracoccus pantotrophus |
sirohydrochlorin-ferrochelatase | - |
Paracoccus pantotrophus |