Cloned (Comment) | Organism |
---|---|
sirB, expression in Escherichia coli BL21(DE3)pLysS, sirA,B,C operon, amino acid sequence | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant SirB | Priestia megaterium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, SDS-PAGE | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Priestia megaterium | SirB is responsible for the final step in siroheme synthesis | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | Priestia megaterium DSM 509 | SirB is responsible for the final step in siroheme synthesis | siroheme + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
strain DSM509 | - |
Priestia megaterium DSM 509 | - |
strain DSM509 | - |
Purification (Comment) | Organism |
---|---|
recombinant SirB | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0034 | - |
pH 8, cobaltochelation of precorrin-2 | Priestia megaterium |
0.337 | - |
pH 8, cobaltochelation of sirohydrochlorin | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precorrin-2 + Co2+ | SirB, much lower specific activity than with sirohydrochlorin | Priestia megaterium | cobalt-precorrin-2 + 2 H+ | - |
? | |
precorrin-2 + Co2+ | SirB, much lower specific activity than with sirohydrochlorin | Priestia megaterium DSM 509 | cobalt-precorrin-2 + 2 H+ | - |
? | |
sirohydrochlorin + Co2+ | SirB, lower specificity for cobalt than for iron | Priestia megaterium | cobalt-sirohydrochlorin + 2 H+ | - |
? | |
sirohydrochlorin + Co2+ | SirB, lower specificity for cobalt than for iron | Priestia megaterium DSM 509 | cobalt-sirohydrochlorin + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt | Priestia megaterium | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | SirB is responsible for the final step in siroheme synthesis | Priestia megaterium | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt | Priestia megaterium DSM 509 | siroheme + 2 H+ | - |
? | |
sirohydrochlorin + Fe2+ | SirB is responsible for the final step in siroheme synthesis | Priestia megaterium DSM 509 | siroheme + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
SirB | Bacillus megaterium | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Priestia megaterium |