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Literature summary for 4.99.1.4 extracted from

  • Schubert, H.L.; Raux, E.; Brindley, A.A.; Leech, H.K.; Wilson, K.S.; Hill, C.P.; Warren, M.J.
    The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase (2002), EMBO J., 21, 2068-2075.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
MET8 gene Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure of Met8p, hanging drop method Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D141A mutant of bifunctional Met8p is completely inactive as both dehydrogenase and ferrochelatase Saccharomyces cerevisiae
G22D mutant of bifunctional Met8p is completely inactive as dehydrogenase, but functions as ferrochelatase Saccharomyces cerevisiae
H237A mutant of bifunctional Met8p is active as both dehydrogenase and ferrochelatase Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Fe2+ Saccharomyces cerevisiae
-
siroheme + H+
-
?
sirohydrochlorin + Fe2+ Saccharomyces cerevisiae Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme siroheme + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae P15807
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Met8p Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae
0.0311
-
wild-type Met8p Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Co2+
-
Saccharomyces cerevisiae cobalt-sirohydrochlorin + 2 H+
-
?
sirohydrochlorin + Fe2+
-
Saccharomyces cerevisiae siroheme + H+
-
?
sirohydrochlorin + Fe2+ Met8p structure, bifunctional Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme, both catalytic activities share a single active site, Asp-141 functions as a general base and plays an essential role in both dehydrogenase and chelatase processes Saccharomyces cerevisiae siroheme + 2 H+
-
?
sirohydrochlorin + Fe2+ Met8p catalyzes the final two steps in the biosynthesis of siroheme involving a beta-NAD+-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield siroheme Saccharomyces cerevisiae siroheme + 2 H+
-
?

Subunits

Subunits Comment Organism
homodimer three structural domains per monomer, domain structure Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Met8p Saccharomyces cerevisiae Saccharomyces cerevisiae
More Met8p is a class 2 chelatase Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae