Literature summary for 4.99.1.11 extracted from

Moore, S.J.; Sowa, S.T.; Schuchardt, C.; Deery, E.; Lawrence, A.D.; Ramos, J.V.; Billig, S.; Birkemeyer, C.; Chivers, P.T.; Howard, M.J.; Rigby, S.E.; Layer, G.; Warren, M.J.
Elucidation of the biosynthesis of the methane catalyst coenzyme F430 (2017), Nature, 543, 78-82 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta pLysS cells	Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sirohydrochlorin + Ni2+	Methanosarcina barkeri	-	Ni-sirohydrochlorin + 2 H+	-	?
sirohydrochlorin + Ni2+	Methanosarcina barkeri Fusaro DSM804	-	Ni-sirohydrochlorin + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina barkeri	-	-	-
Methanosarcina barkeri Fusaro DSM804	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q column chromatography and Superdex 75 gel filtration	Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sirohydrochlorin + Ni2+	-	Methanosarcina barkeri	Ni-sirohydrochlorin + 2 H+	-	?
sirohydrochlorin + Ni2+	-	Methanosarcina barkeri Fusaro DSM804	Ni-sirohydrochlorin + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
CbiXS	-	Methanosarcina barkeri
cfbA	-	Methanosarcina barkeri

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Release 2023.1 (January 2023)