Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aspergillus giganteus |
Crystallization (Comment) | Organism |
---|---|
modeling of structure | Aspergillus giganteus |
Protein Variants | Comment | Organism |
---|---|---|
N54A | mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 65% of wild-type activity with ribosomes and ribosomal loop RNA | Aspergillus giganteus |
N54D | mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 60% of wild-type activity with ribosomes and ribosomal loop RNA | Aspergillus giganteus |
N54Q | mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 24% and 68% of wild-type activity with ribosomes and ribosomal loop RNA, respectively | Aspergillus giganteus |
N54S | mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 28 and 49% of wild-type activity with ribosomes and ribosomal loop RNA, respectively | Aspergillus giganteus |
N54Y | mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. Loss of activity | Aspergillus giganteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus giganteus | P00655 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + H2O | - |
Aspergillus giganteus | ? | - |
? | |
ribosomes + H2O | - |
Aspergillus giganteus | ? | - |
? | |
sarcin/ricin ribosomal loop RNA + H2O | - |
Aspergillus giganteus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
melting temperature, mutants N54D, N54A | Aspergillus giganteus |
45 | - |
melting temperature, mutants N54Q | Aspergillus giganteus |
46 | - |
melting temperature, mutants N54S, N54Y | Aspergillus giganteus |
52 | - |
melting temperature of wild-type | Aspergillus giganteus |