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Literature summary for 4.6.1.23 extracted from

  • Siemer, A.; Masip, M.; Carreras, N.; Garcia-Ortega, L.; Onaderra, M.; Bruix, M.; Del Pozo, A.M.; Gavilanes, J.G.
    Conserved asparagine residue 54 of alpha-sarcin plays a role in protein stability and enzyme activity (2004), Biol. Chem., 385, 1165-1170 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus giganteus

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure Aspergillus giganteus

Protein Variants

Protein Variants Comment Organism
N54A mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 65% of wild-type activity with ribosomes and ribosomal loop RNA Aspergillus giganteus
N54D mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 60% of wild-type activity with ribosomes and ribosomal loop RNA Aspergillus giganteus
N54Q mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 24% and 68% of wild-type activity with ribosomes and ribosomal loop RNA, respectively Aspergillus giganteus
N54S mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. About 28 and 49% of wild-type activity with ribosomes and ribosomal loop RNA, respectively Aspergillus giganteus
N54Y mutation in a conserved residue located in loop 2 of the protein, decrease in melting temperature. Loss of activity Aspergillus giganteus

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus P00655
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + H2O
-
Aspergillus giganteus ?
-
?
ribosomes + H2O
-
Aspergillus giganteus ?
-
?
sarcin/ricin ribosomal loop RNA + H2O
-
Aspergillus giganteus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
melting temperature, mutants N54D, N54A Aspergillus giganteus
45
-
melting temperature, mutants N54Q Aspergillus giganteus
46
-
melting temperature, mutants N54S, N54Y Aspergillus giganteus
52
-
melting temperature of wild-type Aspergillus giganteus