Literature summary for 4.6.1.23 extracted from

Ruggiero, A.; Garcia-Ortega, L.; Ragucci, S.; Russo, R.; Landi, N.; Berisio, R.; Di Maro, A.
Structural and enzymatic properties of Ageritin, a novel metal-dependent ribotoxin-like protein with antitumor activity (2018), Biochim. Biophys. Acta, 1862, 2888-2894 .

Search for more literature for 4.6.1.23

General Stability

General Stability	Organism
in guanidinium hydrochloride, half-completion of denaturing transition occurs at 5.1 M	Cyclocybe aegerita

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	0.6 mM, complete loss of activity	Cyclocybe aegerita

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	ageritin strongly binds magnesium ions with low micromolar affinity, KD value 308 nM	Cyclocybe aegerita
additional information	activity is strongly metal-dependent and completely suppressed in the presence of EDTA	Cyclocybe aegerita

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14400	-	gel filtration	Cyclocybe aegerita

Organism

Organism	UniProt	Comment	Textmining
Cyclocybe aegerita	C0HLG3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	ageritin shows specific ribonucleolytic activity against the ribosomal sarcin-ricin loop in a rabbit reticulocytes assay	Cyclocybe aegerita	?	-	?
rabbit reticulocyte 28S rRNA containing guanosine-adenosine pair + H2O	-	Cyclocybe aegerita	an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-a hydroxy-guanosine-3'-[RNA fragment]	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 16000, SDS-PAGE, 1 * 14803, calculated from sequence	Cyclocybe aegerita

Synonyms

Synonyms	Comment	Organism
AAE3_01767	-	Cyclocybe aegerita

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
78	-	melting temperature	Cyclocybe aegerita

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)