Literature summary for 4.6.1.23 extracted from

Landi, N.; Pacifico, S.; Ragucci, S.; Iglesias, R.; Piccolella, S.; Amici, A.; Di Giuseppe, A.M.A.; Di Maro, A.
Purification, characterization and cytotoxicity assessment of ageritin The first ribotoxin from the basidiomycete mushroom Agrocybe aegerita (2017), Biochim. Biophys. Acta, 1861, 1113-1121 .

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Application

Application	Comment	Organism
medicine	enzyme displays cytotoxity against tumour cells. The ribotoxin dose, able to inhibit by 50% the mitochondrial redox activity of C6 glial cells, is equal to 4.58 microg/ml (0.316 microM). A ribotoxin dose of 8.41 microg/ml inhibits by 50% human SK-N-BE(2)-C neuroblastoma cells and of 9.46 microg/ml U-251 glioma cells	Cyclocybe aegerita

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	gel filtration	Cyclocybe aegerita

Organism

Organism	UniProt	Comment	Textmining
Cyclocybe aegerita	C0HLG3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Saccharomyces cerevisiae rRNA containing guanosine-adenosine pair + H2O	-	Cyclocybe aegerita	an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-a hydroxy-guanosine-3'-[RNA fragment]	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 17000, SDS-PAGE	Cyclocybe aegerita

Synonyms

Synonyms	Comment	Organism
AAE3_01767	-	Cyclocybe aegerita
ageritin	-	Cyclocybe aegerita

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cyclocybe aegerita	isoelectric focusing	-	9.5

General Information

General Information	Comment	Organism
physiological function	purified ageritin is able to inhibit protein synthesis in a rabbit reticulocyte lysate, with an IC50 value of 133 pM	Cyclocybe aegerita

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Release 2023.1 (January 2023)