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Literature summary for 4.6.1.23 extracted from

  • Carreras-Sangra, N.; Tome-Amat, J.; Garcia-Ortega, L.; Batt, C.A.; Onaderra, M.; Martinez-del-Pozo, A.; Gavilanes, J.G.; Lacadena, J.
    Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin alpha-sarcin (2012), Protein Eng. Des. Sel., 25, 425-435.
    View publication on PubMed
Search for more literature for 4.6.1.23

Application

Application Comment Organism
medicine the recombinant fusion enzyme scFvA33alphasarcin shows high specific toxicity against GPA33-positive tumoral cell lines providing scientific evidence to sustain that scFvA33alphasarcin is a good immunotherapeutic candidate against GPA33-positive colon carcinomas Aspergillus giganteus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant extracellular His-tagged enzyme fusion construct scFvA33alphasarcin from expression in Pichia pastoris strain KM71 by nickel affinity chromatgraphy Aspergillus giganteus
the His-tagged enzyme is fused to the single-chain variable fragment (scFv) of the monoclonal antibody that targets GPA33, the construct scFvA33alphasarcin is expressed in Pichia pastoris strain KM71 using the AOX1 promoter, subcloning in Escherichia coli DH5aF cells, and secretion to the medium Aspergillus giganteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, recombinant His-tagged enzyme fusion construct scFvA33alphasarcin, SDS-PAGE Aspergillus giganteus

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
-
-
Aspergillus giganteus MDH18894
-
-
-

Subunits

Subunits Comment Organism
? x * 45000, recombinant His-tagged enzyme fusion construct scFvA33alphasarcin, SDS-PAGE Aspergillus giganteus

Synonyms

Synonyms Comment Organism
alpha-sarcin
-
 Aspergillus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus giganteus

General Information

General Information Comment Organism
additional information the recombinant fusion enzyme scFvA33alphasarcin shows high specific toxicity against GPA33-positive tumoral colon carcinoma cell lines, cellular binding and internalization of recombinant enzyme fusion construct scFvA33alphasarcin, overview Aspergillus giganteus
physiological function the fungal ribotoxin alpha-sarcin is one of the most potent and specific toxins known. It is small, protease-resistant, thermostable and highly efficient towards the inactivation of ribosomes Aspergillus giganteus