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Literature summary for 4.6.1.23 extracted from

  • Garcia-Ortega, L.; Masip, M.; Mancheno, J.M.; Onaderra, M.; Lizarbe, M.A.; Garcia-Mayoral, M.F.; Bruix, M.; Martinez del Pozo, A.; Gavilanes, J.G.
    Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease (2002), J. Biol. Chem., 277, 18632-18639.
    View publication on PubMed
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Application

Application Comment Organism
medicine alpha-sarcin inhibits growth of tumors by inhibition of protein synthesis, protein induces apoptosis in tumor cells after internalization via endocytosis, alpha-sarcin is cytotoxic for normal human cells and for many tumor cell lines, particulary sarcoma and carcinoma, it shows a selective, high level of toxicity against cells with altered or damaged cell membranes, it is a promising candidate for the treatment of cancer and viral infections such as AIDS Aspergillus giganteus

Cloned(Commentary)

Cloned (Comment) Organism
deletion mutant delta(7-22) produced in Escherichia coli Aspergillus giganteus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Aspergillus giganteus
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Organism

Organism UniProt Comment Textmining
Aspergillus giganteus P00655 filamentous fungus, mold
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Purification (Commentary)

Purification (Comment) Organism
deletion mutant DELTA(7-22) Aspergillus giganteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28 S rRNA + H2O
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 Aspergillus giganteus alpha-fragment from the 3' end of 28S rRNA
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 ?
additional information deletion mutant delta(7-22) exhibits activity against naked rRNA and synthetic substrates but lacks the specific ability of the wild-type protein to degrade rRNA in intact ribosomes Aspergillus giganteus ?
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 ?
RNA + H2O hydrolyzes naked RNA at purines in both single-and double-stranded regions Aspergillus giganteus ?
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 ?
rRNA + H2O
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 Aspergillus giganteus ?
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 ?