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Literature summary for 4.6.1.23 extracted from
- Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin (2001), Eur. J. Biochem., 268, 6190-6196.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | alpha-sarcin inhibits growth of tumors by inhibition of protein synthesis, protein induces apoptosis in tumor cells after internalization via endocytosis, alpha-sarcin is cytotoxic for normal human cells and for many tumor cell lines, particulary sarcoma and carcinoma, it shows a selective, high level of toxicity against cells with altered or damaged cell membranes, it is a promising candidate for the treatment of cancer and viral infections such as AIDS | Aspergillus giganteus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Escherichia coli BL21(DE3) | Aspergillus giganteus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R121K | oligonucleotide site-directed mutagenesis | Aspergillus giganteus |
| R121Q | oligonucleotide site-directed mutagenesis | Aspergillus giganteus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
ApA | pH 5.0, 37°C, mutant R121Q | Aspergillus giganteus |  |
| 0.036 | - |
ApA | pH 5.0, 37°C, mutant R121K | Aspergillus giganteus | |
| 0.04 | - |
ApA | pH 5.0, 37°C, wild-type | Aspergillus giganteus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus giganteus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Aspergillus giganteus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus giganteus | - |
filamentous fungus, mold | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Aspergillus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28 S rRNA + H2O | - |
Aspergillus giganteus | alpha-fragment from the 3' end of 28S rRNA | - |
? | |
| ApA + H2O | - |
Aspergillus giganteus | 3'-AMP + adenosine | - |
? | |
| additional information | arginine 121 is a crucial residue for the specific cytotoxic activity of alpha-sarcin | Aspergillus giganteus | ? | - |
? | |
| additional information | acts as a cyclizing ribonuclease | Aspergillus giganteus | ? | - |
? | |
| rRNA + H2O | - |
Aspergillus giganteus | ? | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Aspergillus giganteus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000012 | - |
ApA | pH 5.0, 37°C, mutant R121K | Aspergillus giganteus | |
| 0.000079 | - |
ApA | pH 5.0, 37°C, mutant R121Q | Aspergillus giganteus | |
| 0.00027 | - |
ApA | pH 5.0, 37°C, wild-type | Aspergillus giganteus | |
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Release 2023.1 (January 2023)
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