Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.23 extracted from

  • Lacadena, J.; Martinez del Pozo, A.; Martinez-Ruiz, A.; Perez-Canadillas, J.M.; Bruix, M.; Mancheno, J.M.; Onaderra, M.; Gavilanes, J.G.
    Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin (1999), Proteins, 37, 474-484.
    View publication on PubMed
Search for more literature for 4.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli RB791 Aspergillus giganteus

Protein Variants

Protein Variants Comment Organism
E96Q mutagenesis variant protein Aspergillus giganteus
H137Q mutagenesis variant protein Aspergillus giganteus
H50/137Q mutagenesis variant protein Aspergillus giganteus
H50/137Q/E96Q mutagenesis variant protein Aspergillus giganteus
H50Q mutagenesis variant protein Aspergillus giganteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 ApA pH 7.0, 37°C, mutant H50Q Aspergillus giganteus
0.04
-
 ApA pH 7.0 or pH 5.0, 37°C Aspergillus giganteus
0.045
-
 ApA pH 5.0, 37°C, mutant H50Q Aspergillus giganteus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Aspergillus giganteus
-
 ?
-
 ?
rRNA + H2O Aspergillus giganteus MDH 18894
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
 MDH 18894
-
Aspergillus giganteus
-
 filamentous fungus, mold
-
Aspergillus giganteus MDH 18894
-
 MDH 18894
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus giganteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ApA + H2O
-
 Aspergillus giganteus 3'-AMP + adenosine
-
 ?
ApA + H2O
-
 Aspergillus giganteus MDH 18894 3'-AMP + adenosine
-
 ?
additional information acts as a cyclizing ribonuclease Aspergillus giganteus ?
-
 ?
additional information acts as a cyclizing ribonuclease Aspergillus giganteus MDH 18894 ?
-
 ?
rRNA + H2O
-
 Aspergillus giganteus ?
-
 ?
rRNA + H2O
-
 Aspergillus giganteus MDH 18894 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000014
-
 ApA pH 7.0, 37°C, mutant H50Q Aspergillus giganteus
0.000016
-
 ApA pH 5.0, 37°C, mutant H50Q Aspergillus giganteus
0.0001
-
 ApA pH 7.0, 37°C Aspergillus giganteus
0.0002
-
 ApA pH 5.0, 37°C Aspergillus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus giganteus

pH Range

pH Minimum pH Maximum Comment Organism
3 8.5
-
 Aspergillus giganteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8.5
-
 Aspergillus giganteus