Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nitric oxide | - |
Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
the beta1 subunit of soluble guanylyl cyclase is expressed in Escherichia coli BL21 (DE3) pLysS cells | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 7.0, and 0.7 M ammonium sulfate | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19500 | - |
1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
38500 | - |
1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Rattus norvegicus | - |
3',5'-cyclic-GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P20595 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Rattus norvegicus | 3',5'-cyclic-GMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Rattus norvegicus |
sGCbeta1 | beta1 subunit of soluble guanylyl cyclase | Rattus norvegicus |
soluble guanylyl cyclase | - |
Rattus norvegicus |