Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanylyl cyclase activating protein 1 | GCAP1, a Ca2+/Mg2+-binding protein, after substitution of Ca2+ by Mg2+ in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. Metal binding in EF-hand 2 is crucial for GCAP1 attachment to RetGC1, while in EF-hand 3 it is less critical, although it enhances the efficiency of the GCAP1 docking on the target enzyme. Metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type GFP-tagged RetGC1 and commercially available dsRet-tagged RetGC1 in HEK-293 cells, co-expression with either fluorescently tagged or non-tagged guanylyl cyclase activating protein 1, with inactivated metal binding in individual EF-hands, in cell membranes. The uniform cellular distribution of GCAP1, also in the nucleus, drastically changes when the cells express both GCAP1-GFP and RetGC1, GCAP1 is then depleted from the nuclei and only observed in the cytoplasm of the cells, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D100N/D102G | the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme | Homo sapiens |
E75Q/E111Q/E155Q | the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanylyl cyclase activating protein 1 | GCAP1, a Ca2+/Mg2+-binding protein, metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Homo sapiens | - |
3',5'-cyclic GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | dsRetGC1 | Homo sapiens | - |
retina | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Homo sapiens | 3',5'-cyclic GMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal portion of RetGC1 contains its regulatory and catalytic domains | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
photoreceptor guanylyl cyclase | - |
Homo sapiens |
RetGC1 | - |
Homo sapiens |
retinal guanylyl cyclase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |