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Literature summary for 4.6.1.2 extracted from

  • Peshenko, I.V.; Olshevskaya, E.V.; Dizhoor, A.M.
    Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands (2008), J. Biol. Chem., 283, 21747-21757.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
guanylyl cyclase activating protein 1 GCAP1, a Ca2+/Mg2+-binding protein, after substitution of Ca2+ by Mg2+ in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. Metal binding in EF-hand 2 is crucial for GCAP1 attachment to RetGC1, while in EF-hand 3 it is less critical, although it enhances the efficiency of the GCAP1 docking on the target enzyme. Metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type GFP-tagged RetGC1 and commercially available dsRet-tagged RetGC1 in HEK-293 cells, co-expression with either fluorescently tagged or non-tagged guanylyl cyclase activating protein 1, with inactivated metal binding in individual EF-hands, in cell membranes. The uniform cellular distribution of GCAP1, also in the nucleus, drastically changes when the cells express both GCAP1-GFP and RetGC1, GCAP1 is then depleted from the nuclei and only observed in the cytoplasm of the cells, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
D100N/D102G the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme Homo sapiens
E75Q/E111Q/E155Q the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
guanylyl cyclase activating protein 1 GCAP1, a Ca2+/Mg2+-binding protein, metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Homo sapiens
-
3',5'-cyclic GMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation dsRetGC1 Homo sapiens
-
retina
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Homo sapiens 3',5'-cyclic GMP + diphosphate
-
?

Subunits

Subunits Comment Organism
More the C-terminal portion of RetGC1 contains its regulatory and catalytic domains Homo sapiens

Synonyms

Synonyms Comment Organism
photoreceptor guanylyl cyclase
-
Homo sapiens
RetGC1
-
Homo sapiens
retinal guanylyl cyclase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens