Literature summary for 4.6.1.2 extracted from

Alfonzo, M.J.; de Aguilar, E.P.; de Murillo, A.G.; de Villarroel, S.S.; de Alfonzo, R.G.; Borges, A.; de Becemberg, I.L.
Characterization of a G protein-coupled guanylyl cyclase-B receptor from bovine tracheal smooth muscle (2006), J. Recept. Signal Transduct. Res., 26, 269-297.

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Activating Compound

Activating Compound	Comment	Organism	Structure
atrial NP 1-28	-	Bos taurus
C-type NP 1-22	-	Bos taurus
C-type NP 1-53	activation in a dose-dependent manner	Bos taurus
GTPgammaS	increases activity to 58%	Bos taurus
mastoparan	most powerful activator, activation in a dose-dependent manner	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	80% inhibition at 0.1 mM, affects C-type NP 1-53 stimulation of the enzyme, in the presence of Mn2+ inhibition of mastoparan-induced activation of the enzyme	Bos taurus
GTPgammaS	inhibits mastoparan-induced activation	Bos taurus
NaCl	80% inhibition at 0.2 mM, affects C-type NP 1-53 stimulation of the enzyme	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	GTP	octylglucoside solubilized enzyme, Mn2+-supported enzyme	Bos taurus
0.14	-	GTP	native enzyme, Mn2+-supported enzyme	Bos taurus
0.4	-	GTP	native enzyme, Mg2+-supported enzyme	Bos taurus
0.401	-	GTP	octylglucoside solubilized enzyme, Mg2+-supported enzyme	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bos taurus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates up to 2 mM, in the presence of small amounts of Mn2+ activation increases dramatically	Bos taurus
Mn2+	-	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
tracheal smooth muscle	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP	-	Bos taurus	3',5'-cyclic GMP + diphosphate	-	?
GTPgammaS	-	Bos taurus	?	-	?
additional information	GMPNH-P, GMPCH-P and N-methylanthraniloyl 2'-GTP are poor substrates	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
CNP-sensitive guanylyl cyclase	-	Bos taurus
membrane-bound guanylyl cyclase	-	Bos taurus
mGc	-	Bos taurus
NP-GC	-	Bos taurus
NPR-B	-	Bos taurus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.07	-	-	Bos taurus	ATP
0.07	-	-	Bos taurus	NaCl

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Release 2023.1 (January 2023)