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Literature summary for 4.6.1.2 extracted from

  • Lee, Y.C.; Martin, E.; Murad, F.
    Human recombinant soluble guanylyl cyclase: expression, purification, and regulation (2000), Proc. Natl. Acad. Sci. USA, 97, 10763-10768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
3-(5'-hydroxymethyl-2'furyl)-1-benzyl-indazole 0.1 mM, approx. 50fold stimulation, more than 2000fold stimulation in the presence of 0.1 mM sodium prusside Homo sapiens
NO
-
Homo sapiens
sodium nitroprusside 0.1 mM, 580fold stimulation, NO donor Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of alpha1 and beta1 subunits of sGC in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one 0.1 mM, complete inhibition Homo sapiens
3',5'-cyclic GMP 20 mM, 20-30% competitive inhibition Homo sapiens
diphosphate competitive inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
GTP recombinant sGC Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
gel filtration, recombinant His-tagged sGC Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Homo sapiens
-
3',5'-cyclic GMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02108
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant sGC Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0056
-
basal sGC activity Homo sapiens
3.25
-
sodium nitroprusside activated sGC activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Homo sapiens 3',5'-cyclic GMP + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer alpha1,beta1, 0.9 equivalents of heme per sGC subunit Homo sapiens

Synonyms

Synonyms Comment Organism
sGC
-
Homo sapiens
soluble guanylyl cyclase
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
diphosphate
-
Homo sapiens