Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-(5'-hydroxymethyl-2'furyl)-1-benzyl-indazole | 0.1 mM, approx. 50fold stimulation, more than 2000fold stimulation in the presence of 0.1 mM sodium prusside | Homo sapiens | |
NO | - |
Homo sapiens | |
sodium nitroprusside | 0.1 mM, 580fold stimulation, NO donor | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of alpha1 and beta1 subunits of sGC in Sf9 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one | 0.1 mM, complete inhibition | Homo sapiens | |
3',5'-cyclic GMP | 20 mM, 20-30% competitive inhibition | Homo sapiens | |
diphosphate | competitive inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
GTP | recombinant sGC | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
gel filtration, recombinant His-tagged sGC | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Homo sapiens | - |
3',5'-cyclic GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q02108 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant sGC | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0056 | - |
basal sGC activity | Homo sapiens |
3.25 | - |
sodium nitroprusside activated sGC activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Homo sapiens | 3',5'-cyclic GMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha1,beta1, 0.9 equivalents of heme per sGC subunit | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Homo sapiens |
soluble guanylyl cyclase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
diphosphate | - |
Homo sapiens |