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Literature summary for 4.6.1.19 extracted from
- Secretory acid ribonucleases from tomato, Lycopersicon esculentum Mill (2001), Methods Enzymol., 341, 351-368.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | 25 mM sodium citrate buffer results in 170% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 | Solanum lycopersicum |  |
| additional information | enzymes are induced by phosphate starvation | Solanum lycopersicum | |
| phosphate | 25 mM sodium phosphate buffer results in 153% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 | Solanum lycopersicum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
- |
Zinnia elegans |
| enzymes are S-gene products, cloning of RNases Le and LX, DNA and amino acid sequence determination and analysis | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoic acid | 80% inhibition of RNase LE at 1 mM | Solanum lycopersicum | |
| Ag+ | complete inhibition at 10 mM | Solanum lycopersicum | |
| Al3+ | complete inhibition at 10 mM | Solanum lycopersicum | |
| Cu2+ | complete inhibition at 10 mM | Solanum lycopersicum | |
| Hg2+ | complete inhibition at 10 mM | Solanum lycopersicum | |
| additional information | no effect of 2.5 mM EDTA, no product inhibition | Solanum lycopersicum | |
| Zn2+ | complete inhibition at 10 mM | Solanum lycopersicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | RNase LX | Solanum lycopersicum | 5783 | - |
| extracellular | RNase LE | Solanum lycopersicum | - |
- |
| microsome | RNase LX | Solanum lycopersicum | - |
- |
| vacuole | RNases LV1-3 | Solanum lycopersicum | 5773 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | RNases LE, LX and LV-3 do not require divalent cations | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Solanum lycopersicum | besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
| additional information | Arabidopsis thaliana | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
| additional information | Zinnia elegans | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
| additional information | Momordica charantia | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
| additional information | Nicotiana glutinosa | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Momordica charantia | - |
bitter gourd, RNase MC1 | - |
| Nicotiana glutinosa | Q9SSV1 | RNase NGR3 | - |
| Solanum lycopersicum | - |
1 extracellular, 3 vacuolar and 1 microsomal enzyme forms | - |
| Zinnia elegans | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzymes contain secretory targeting signals, RNase LX contains an ER retention signal sequence | Solanum lycopersicum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Momordica charantia |
- |
Nicotiana glutinosa |
| preparation of microsomes, protoplasts, vacuoles, cell culture supernatant, purification of RNase LE, LX, and LV-1-3, different methods, 1800-4400fold, overview | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | phosphate-starved | Solanum lycopersicum | - |
| culture medium | from phosphate-starved cells | Solanum lycopersicum | - |
| leaf | - |
Nicotiana glutinosa | - |
| seed | - |
Momordica charantia | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzymes, at least 2 months, no loss of activity | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-cyclic NMP + H2O | slower side reaction, intrinsic cyclic nucleotide phosphodiesterase activity | Solanum lycopersicum | 3'(2')-NMP | - |
? | |
| additional information | besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions | Solanum lycopersicum | ? | - |
? | |
| additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Zinnia elegans | ? | - |
? | |
| additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Momordica charantia | ? | - |
? | |
| additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Nicotiana glutinosa | ? | - |
? | |
| additional information | enzymes show preference for release of guanine mononucleotides from diribonucleoside monophosphates or RNA, preference in descending order is G, A, U, C | Solanum lycopersicum | ? | - |
? | |
| nucleotidyl-3',5'-nuceotide + H2O | determination of base specificity | Solanum lycopersicum | 2',3'-cyclic NMP + 3'(2')-NMP | - |
? | |
| RNA + H2O | high-molecular weight fraction of RNA from Torula yeast, typeII-S | Solanum lycopersicum | 2',3'-cyclic NMP + 3'(2')-NMP | 2',3'-cyclic NMPs are are further hydrolyzed in a side reaction | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | comparison of primary, secondary, and tertiary structures, overview | Arabidopsis thaliana |
| More | comparison of primary, secondary, and tertiary structures, overview | Solanum lycopersicum |
| More | comparison of primary, secondary, and tertiary structures, overview | Zinnia elegans |
| More | comparison of primary, secondary, and tertiary structures, overview | Momordica charantia |
| More | comparison of primary, secondary, and tertiary structures, overview | Nicotiana glutinosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzymes are S-like ribonucleases | Arabidopsis thaliana |
| More | enzymes are S-like ribonucleases | Zinnia elegans |
| More | enzymes are S-like ribonucleases | Nicotiana glutinosa |
| More | enzymes belong to the RNase 1-type and are S-like RNases | Solanum lycopersicum |
| More | enzymes is a S-like ribonuclease | Momordica charantia |
| ribonuclease LE | - |
Solanum lycopersicum |
| RNase LE | - |
Solanum lycopersicum |
| RNase LV-1 | - |
Solanum lycopersicum |
| RNase LV-2 | - |
Solanum lycopersicum |
| RNase LV-3 | - |
Solanum lycopersicum |
| RNase LX | - |
Solanum lycopersicum |
| RNase MC1 | - |
Momordica charantia |
| RNase NGR3 | - |
Nicotiana glutinosa |
| RNase NW | - |
Nicotiana glutinosa |
| RNS1 | - |
Arabidopsis thaliana |
| RNS2 | - |
Arabidopsis thaliana |
| RNS3 | - |
Arabidopsis thaliana |
| ZRNase I | - |
Zinnia elegans |
| ZRNase II | - |
Zinnia elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
5 min, different pH values and ionic strengths, complete inactivation | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | acidic optimum with yeast RNA as a substrate | Solanum lycopersicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Solanum lycopersicum | about, RNase LV-3, isoelectric focusing | - |
4 |
| Solanum lycopersicum | about, RNase LV-1, isoelectric focusing | - |
4.9 |
| Solanum lycopersicum | about, RNase LV-2, isoelectric focusing | - |
4.9 |
| Solanum lycopersicum | about, RNase LX, isoelectric focusing | - |
4.9 |
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Release 2023.1 (January 2023)
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