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Literature summary for 4.6.1.19 extracted from
- RNases, I, II, and IV of Escherichia coli (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15B, 501-515.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | polyamines stimulate | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DNA | denatured | Escherichia coli |  |
| MgCl2 | slight | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| ribosome | - |
Escherichia coli | 5840 | - |
| ribosome | 30S ribosomes, selectively | Aspergillus phoenicis | 5840 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | enhances activity | Escherichia coli | |
| NaCl | enhances activity | Escherichia coli | |
| NaF | enhances activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11710 | - |
amino acid sequence | Aspergillus phoenicis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus phoenicis | - |
- |
- |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, after dialysis against water | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | - |
Escherichia coli | 3'-phosphomononucleotides | - |
? | |
| RNA + H2O | - |
Aspergillus phoenicis | 3'-phosphomononucleotides | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
pH 3.1: 15 min, pH 8.8: complete loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | pH 7.5, pH 8.5: 85% of activity maximum | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | 8 | 4°C, for at least 4 days | Escherichia coli |
| 9.8 | - |
4°C, 8% loss of activity after 4 days | Escherichia coli |
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