BRENDA - Enzyme Database show
show all sequences of 4.6.1.14

Glycosylphosphatidylinositol-specific phospholipase C regulates transferrin endocytosis in the African trypanosome

Subramanya, S.; Hardin, C.F.; Steverding, D.; Mensa-Wilmot, K.; Biochem. J. 417, 685-694 (2009)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
Q81L
catalytically inactive mutant
Trypanosoma brucei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
GPI-PLC is not detected at endosomes
Trypanosoma brucei
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Trypanosoma brucei
GPI-PLC is a signalling enzyme, and DAG is a second messenger for GPI-PLC. Its enzyme activity is important for stimulating uptake of transferrin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei
-
427 and RUMP528
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
bloodstream-form Trypanosoma brucei RUMP528 (GPI-PLC-/-)
Trypanosoma brucei
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
GPI-PLC is a signalling enzyme, and DAG is a second messenger for GPI-PLC. Its enzyme activity is important for stimulating uptake of transferrin
690839
Trypanosoma brucei
?
-
-
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Q81L
catalytically inactive mutant
Trypanosoma brucei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
GPI-PLC is not detected at endosomes
Trypanosoma brucei
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Trypanosoma brucei
GPI-PLC is a signalling enzyme, and DAG is a second messenger for GPI-PLC. Its enzyme activity is important for stimulating uptake of transferrin
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
bloodstream-form Trypanosoma brucei RUMP528 (GPI-PLC-/-)
Trypanosoma brucei
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
GPI-PLC is a signalling enzyme, and DAG is a second messenger for GPI-PLC. Its enzyme activity is important for stimulating uptake of transferrin
690839
Trypanosoma brucei
?
-
-
-
-
Other publictions for EC 4.6.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747647
Van Veen
Negative regulation of urokin ...
Homo sapiens
eLife
6
e23649
2017
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730795
Sunter
Determinants of GPI-PLC locali ...
Trypanosoma brucei, Trypanosoma brucei 427, Trypanosoma congolense
PLoS Pathog.
9
e1003566
2013
-
-
-
-
-
2
-
-
2
-
-
-
-
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
708444
Subramanya
Trypanosoma brucei: reduction ...
Trypanosoma brucei
Exp. Parasitol.
125
222-229
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
710429
Davies
The alpha2delta subunits of vo ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
107
1654-1659
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715721
Xing
Effects of membrane cholestero ...
Mus musculus
J. Cell. Physiol.
226
2350,235-9
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
690839
Subramanya
Glycosylphosphatidylinositol-s ...
Trypanosoma brucei
Biochem. J.
417
685-694
2009
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691540
Mueller
Hydrogen peroxide-induced tran ...
Rattus norvegicus
Br. J. Pharmacol.
154
901-913
2008
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662808
Gruszynski
Regulation of surface coat exc ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
147
211-223
2006
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679738
Subramanya
Regulated cleavage of intracel ...
Trypanosoma brucei, Trypanosoma brucei 427
FEBS J.
273
2110-2126
2006
-
-
1
-
11
-
-
-
2
-
-
-
-
13
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
661039
Müller
Regulation of lipid raft prote ...
Bacillus cereus, Rattus norvegicus, Trypanosoma brucei
Biochem. Pharmacol.
69
761-780
2005
-
-
-
-
-
-
7
-
1
-
-
3
-
4
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
7
1
-
1
-
-
3
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662276
Zheng
Endosomes, glycosomes, and gly ...
Trypanosoma brucei
J. Biol. Chem.
279
42106-42113
2004
-
-
1
-
8
-
-
-
2
-
-
1
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648771
Müller
Insulin-like signaling in yeas ...
Rattus norvegicus, Saccharomyces cerevisiae
Biochemistry
39
1475-1488
2000
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648782
Armah
Tetramerization of glycosylpho ...
Trypanosoma brucei
J. Biol. Chem.
275
19334-19342
2000
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648783
Paturiaux-Hanocq
A role for the dynamic acylati ...
Trypanosoma brucei
J. Biol. Chem.
275
12147-12155
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648779
Rashid
Roles of Gln81 and Cys80 in ca ...
Trypanosoma brucei
Eur. J. Biochem.
264
914-920
1999
-
-
-
-
11
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648780
Armah
S-myristoylation of a glycosyl ...
Trypanosoma brucei
J. Biol. Chem.
274
5931-5938
1999
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
648781
Ochatt
Conditional expression of glyc ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
103
35-48
1999
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648778
Carrington
The properties and function of ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
91
153-164
1998
-
-
1
-
10
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648775
Webb
The GPI-phospholipase C of Try ...
Trypanosoma brucei
J. Cell. Biol.
139
103-114
1997
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648777
Carnall
Mutagenesis study of the glyco ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
90
423-432
1997
-
-
1
-
9
-
2
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648774
Rolin
Simultaneous but independent a ...
Trypanosoma brucei
J. Biol. Chem.
271
10844-10852
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648776
Buetikofer
Phosphatidylinositol hydrolysi ...
Arachis hypogaea, Trypanosoma brucei
J. Biol. Chem.
271
15533-15541
1996
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648769
Morris
Glycan requirements of glycosy ...
Trypanosoma brucei
J. Biol. Chem.
270
2517-2524
1995
1
-
-
-
-
-
15
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648773
Mensa-Wilmot
Purification and use of recomb ...
Trypanosoma brucei
Methods Enzymol.
250
641-655
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648772
Müller
Stimulation of a glycosyl-phos ...
Rattus norvegicus
J. Cell Biol.
126
1267-1276
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648770
Butikofer
Partial purification and chara ...
Arachis hypogaea
J. Biol. Chem.
268
17794-17802
1993
1
-
-
-
-
-
9
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
648768
Fouchier
Identification of glycosylphos ...
Mus musculus
Biochem. J.
269
321-327
1990
3
-
-
-
-
-
5
-
4
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
5
-
-
4
-
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
135294
Fox
Purification and characterizat ...
Trypanosoma brucei, Trypanosoma brucei 427
J. Biol. Chem.
261
15767-15771
1986
2
-
-
-
-
1
3
-
2
3
1
2
-
12
-
-
1
-
-
-
1
-
9
1
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
1
-
3
-
-
2
3
1
2
-
-
-
1
-
-
1
-
9
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
648767
Hereld
A phospholipase C from Trypano ...
Trypanosoma brucei
J. Biol. Chem.
261
13813-13819
1986
3
-
-
-
-
-
10
-
1
1
2
-
-
1
-
-
1
-
-
-
1
3
6
1
1
-
2
-
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
10
-
-
1
1
2
-
-
-
-
1
-
-
1
3
6
1
1
-
2
-
1
1
-
-
-
-
-
-
-
-