Literature summary for 4.6.1.14 extracted from

Carrington, M.; Carnall, N.; Crow, M.S.; Gaud, A.; Redpath, M.B.; Wasunna, C.L.; Webb, H.
The properties and function of the glycosylphosphatidylinositol-phospholipase C in Trypanosoma brucei (1998), Mol. Biochem. Parasitol., 91, 153-164.

Search for more literature for 4.6.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the PLC elimination construct in Trypanosoma brucei	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
C184S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C24S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C269S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C270S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C273S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C332S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C347S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
C80S	fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid	Trypanosoma brucei
H34Q	enzyme is totally inactive	Trypanosoma brucei
additional information	PLC elimination construct, which yielded in a PLC null-mutant	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0008	-	Acetylcholinesterase	-	Trypanosoma brucei
0.037	-	phosphatidylinositol	-	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetylcholinesterase	-	Trypanosoma brucei	soluble acetylcholinesterase + 1,2-diacylglycerol	-	?
phosphatidylinositol	-	Trypanosoma brucei	inositol 1,2-cyclic phosphate + inositol phosphate	-	?
variant-surface-glycoprotein	-	Trypanosoma brucei	1,2-didecanoylglycerol + soluble variant-surface-glycoprotein	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)