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Literature summary for 4.6.1.14 extracted from
- Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei (1997), Mol. Biochem. Parasitol., 90, 423-432.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Trypanosoma brucei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C184S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C24S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C269S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C270S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C273S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C332S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C347S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C80S | fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| H34Q | enzyme is totally inactive | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuriphenylsulfonic acid | - |
Trypanosoma brucei |  |
| Zn2+ | - |
Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | 0.0007 | Variant-surface-glycoprotein | pH 8 | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.45 | - |
- |
Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| variant-surface-glycoprotein | - |
Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | - |
? | |
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Release 2023.1 (January 2023)
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