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Literature summary for 4.6.1.14 extracted from

  • Hereld, D.; Krakow, J.L.; Bangs, J.D.; Hart, G.W.; Englund, P.T.
    A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein (1986), J. Biol. Chem., 261, 13813-13819.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT activation, 0.025 M Trypanosoma brucei
EDTA activation, 5 mM Trypanosoma brucei
EGTA activation, 5 mM Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak, 5 mM Trypanosoma brucei
KCl 0.125 M Trypanosoma brucei
Mg2+ weak, 5 mM Trypanosoma brucei
additional information no inhibition by IAA Trypanosoma brucei
NaCl 0.125 M Trypanosoma brucei
NEM 5 mM Trypanosoma brucei
NH4Cl 0.125 M Trypanosoma brucei
p-chloromercuriphenylsulfonic acid strong, 5 mM Trypanosoma brucei
sulfhydryl reagents
-
Trypanosoma brucei
Zn2+ strong, 5 mM Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Trypanosoma brucei 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
1 * 37000, SDS-PAGE Trypanosoma brucei
47000
-
gel filtration, sedimentation equilibrium centrifugation, in the presence of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
ILTat 1.3, isolated from blood of Swiss mice or Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
solubilized by n-octyl glucoside Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11000000
-
concentrated CM-Sephadex pool Trypanosoma brucei

Storage Stability

Storage Stability Organism
-70°C, in crude membranes, 6 months Trypanosoma brucei
-70°C, purified enzyme preparation, at least 2 months Trypanosoma brucei
4°C, 30% loss within 5 days, 10% loss of activity in the presence of 50% glycerol Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dimyristoyl-sn-phosphatidylinositol + H2O poor substrate Trypanosoma brucei ?
-
?
lipid A biological precursor of variant-surface-glycoprotein glycolipid Trypanosoma brucei ?
-
?
additional information no substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidylinositol Trypanosoma brucei ?
-
?
variant-surface-glycoprotein highly specific Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein i.e. 1,2-dimyristoylglycerol ?
variant-surface-glycoprotein substrate is tethered to cell membrane by glycolipid moiety containing 1,2-dimyristoyl-sn-phosphatidylinositol Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein i.e. 1,2-dimyristoylglycerol ?
variant-surface-glycoprotein phospholipase C-type hydrolysis Trypanosoma brucei 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein i.e. 1,2-dimyristoylglycerol ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trypanosoma brucei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
10 min, stable Trypanosoma brucei
50
-
10 min, inactivation Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Trypanosoma brucei

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 about half-maximal activity at pH 6.5 and 9.5, little or no activity below pH 6 Trypanosoma brucei