Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | activation, 0.025 M | Trypanosoma brucei | |
EDTA | activation, 5 mM | Trypanosoma brucei | |
EGTA | activation, 5 mM | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak, 5 mM | Trypanosoma brucei | |
KCl | 0.125 M | Trypanosoma brucei | |
Mg2+ | weak, 5 mM | Trypanosoma brucei | |
additional information | no inhibition by IAA | Trypanosoma brucei | |
NaCl | 0.125 M | Trypanosoma brucei | |
NEM | 5 mM | Trypanosoma brucei | |
NH4Cl | 0.125 M | Trypanosoma brucei | |
p-chloromercuriphenylsulfonic acid | strong, 5 mM | Trypanosoma brucei | |
sulfhydryl reagents | - |
Trypanosoma brucei | |
Zn2+ | strong, 5 mM | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Trypanosoma brucei | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000, SDS-PAGE | Trypanosoma brucei |
47000 | - |
gel filtration, sedimentation equilibrium centrifugation, in the presence of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
ILTat 1.3, isolated from blood of Swiss mice or Wistar rats | - |
Purification (Comment) | Organism |
---|---|
solubilized by n-octyl glucoside | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11000000 | - |
concentrated CM-Sephadex pool | Trypanosoma brucei |
Storage Stability | Organism |
---|---|
-70°C, in crude membranes, 6 months | Trypanosoma brucei |
-70°C, purified enzyme preparation, at least 2 months | Trypanosoma brucei |
4°C, 30% loss within 5 days, 10% loss of activity in the presence of 50% glycerol | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dimyristoyl-sn-phosphatidylinositol + H2O | poor substrate | Trypanosoma brucei | ? | - |
? | |
lipid A | biological precursor of variant-surface-glycoprotein glycolipid | Trypanosoma brucei | ? | - |
? | |
additional information | no substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidylinositol | Trypanosoma brucei | ? | - |
? | |
variant-surface-glycoprotein | highly specific | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? | |
variant-surface-glycoprotein | substrate is tethered to cell membrane by glycolipid moiety containing 1,2-dimyristoyl-sn-phosphatidylinositol | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? | |
variant-surface-glycoprotein | phospholipase C-type hydrolysis | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
10 min, stable | Trypanosoma brucei |
50 | - |
10 min, inactivation | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Trypanosoma brucei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | about half-maximal activity at pH 6.5 and 9.5, little or no activity below pH 6 | Trypanosoma brucei |