Crystallization (Comment) | Organism |
---|---|
wild-type and D274N mutant PI-PLC | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
D274A | catalytic aspartate mutation, 0.005% of wild-type activity, no activation by exogenous anions | Bacillus thuringiensis |
D274E | catalytic aspartate mutation, 50% of wild-type activity, no activation by chloride ions | Bacillus thuringiensis |
D274G | catalytic aspartate mutation, activation of mutant PI-PLC by exogenous anions, e.g. Cl- | Bacillus thuringiensis |
D274N | catalytic aspartate mutation, 40fold decreased activity compared with wild-type enzyme, no activation by chloride ions | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Bacillus thuringiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | 1 M, 2-3fold activation of wild-type PI-PLC | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant PI-PLC | Bacillus thuringiensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol | mechanism | Bacillus thuringiensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol | catalyzes the cleavage of the phosphorus-oxygen bond in phosphatidylinositol, catalytic role of aspartate in a short strong hydrogen bond of the Asp274-His32 catalytic dyad, catalytic mechanism, active site structure | Bacillus thuringiensis | 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | wild-type PI-PLC, in the absence of 1 M Cl- | Bacillus thuringiensis |
7 | 8 | wild-type PI-PLC, in the presence of 1 M Cl- | Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence study of wild-type and mutant PI-PLC | Bacillus thuringiensis |